QBEmax是一个序列排列和内部保护的碱基编辑器

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2025-04-21 DOI:10.1038/s41587-025-02641-9

Jiacheng Hu, Mengyue Guo, Qiang Gao, He Jia, Mingyang He, Zhiwei Wang, Lina Guo, Guanwen Liu, Quan Gao, Kevin Tianmeng Zhao

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引用次数: 0

摘要

胞嘧啶碱基编辑器（CBEs）显示出多基因敲除应用的前景，但不纯编辑、缺失和脱靶仍然经常发生。我们在这里描述了QBEmax，它具有高效率，低indel和脱靶性和高产品纯度，高达99.8%的编辑由C-to-T组成。通过分子动力学建模，QBEmax呈现为一个紧凑和稳定的碱基编辑器，保护碱基免受不希望的修复过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

QBEmax is a sequence-permuted and internally protected base editor

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QBEmax is a sequence-permuted and internally protected base editor

Cytosine base editors (CBEs) show promise for multiplex gene knockout applications, but impure edits, indels and off-targets still frequently occur. We describe here QBEmax, which exhibits high efficiency, low indel and off-targets and high product purity with up to 99.8% of edits comprised of C-to-T. Through molecular dynamic modeling, QBEmax presents as a compact and stable base editor that shields protected bases from undesired repair processes.

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

期刊介绍： Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.