小型反刍动物精子的低温冷冻和精子冷冻能力检测。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4406-5_12

Patricia Peris-Frau, Silvia Gimeno-Martos

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引用次数: 0

摘要

精子冷冻保存是保存有价值的雄性或濒危物种的遗传和表观遗传物质不可缺少的工具。在这里，我们描述了最常用于传统冷冻保存小反刍动物精子的方案，考虑到精子样本的来源（附睾与射精精子）和物种之间的差异。此外，由于精子冷冻保存的主要缺点之一是由于相当数量的精子发生了类似于失能的变化，从而降低了生存能力和生育能力，因此本文解释了检测小反刍动物精子冷冻失能的不同方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cryopreservation of Small Ruminant Sperm and Detection of Sperm Cryocapacitation.

Sperm cryopreservation is an indispensable tool for preserving the genetic and epigenetic material of valuable males or endangered species. Here, we describe the protocols most frequently used for conventional cryopreservation of small ruminant sperm, considering differences between the origin of the sperm sample (epididymal vs. ejaculated sperm) and species. In addition, since one of the major drawbacks of sperm cryopreservation is the reduction of viability and fertility due to capacitation-like changes in a considerable amount of sperm, different methods to detect sperm cryocapacitation in small ruminants are explained.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.