Cédric Garcia, Fanny Vardon-Bounes, Baptiste Compagnon, Céline Guilbeau-Frugier, Sophie Voisin, Jean-Baptiste Rieu, Véronique De Mas, Bernard Payrastre, Agnès Ribes
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Bone marrow sampling, aimed to explore thrombocytopenia's etiology by cytology, allowed us to document bone marrow MK behavior.</p><p><strong>Results: </strong>A reduction in bone marrow cellularity and a decrease in the megakaryocytic lineage, were observed suggesting a central component of the thrombocytopenia. Bone marrow MK exhibited significantly increased emperipolesis and vacuolization. Moreover, transmission electron microscopy pointed to the presence of viral particles inside bone marrow MK. Immunolabeling confirmed the presence of two SARS-CoV-2 proteins, spike and Orf3a, as well as double-stranded RNA suggesting a potential viral replication cycle.</p><p><strong>Conclusion: </strong>In this series of COVID-19 patients with thrombocytopenia we report the presence of SARS-CoV-2 in bone marrow MK as well as a decrease in MK lineage and an increase in emperipolesis.</p>","PeriodicalId":17326,"journal":{"name":"Journal of Thrombosis and Haemostasis","volume":" ","pages":""},"PeriodicalIF":5.5000,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of SARS-CoV-2 in bone marrow megakaryocytes and elevated emperipolesis in COVID-19 patients with thrombocytopenia.\",\"authors\":\"Cédric Garcia, Fanny Vardon-Bounes, Baptiste Compagnon, Céline Guilbeau-Frugier, Sophie Voisin, Jean-Baptiste Rieu, Véronique De Mas, Bernard Payrastre, Agnès Ribes\",\"doi\":\"10.1016/j.jtha.2025.03.036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Thrombocytopenia and altered platelet activation correlate with COVID-19 severity and mortality. COVID-19 patients have modifications of the platelet and blood circulating megakaryocytes (MK) transcriptome. Our objective was to explore the features of bone marrow MK, which remain poorly characterized in SARS-CoV-2 infected patients, particularly those with thrombocytopenia.</p><p><strong>Methods: </strong>In this case series study, we analyzed bone marrow samples from a series of 11 COVID-19 patients with thrombocytopenia admitted to the intensive care unit for acute respiratory distress syndrome. Bone marrow sampling, aimed to explore thrombocytopenia's etiology by cytology, allowed us to document bone marrow MK behavior.</p><p><strong>Results: </strong>A reduction in bone marrow cellularity and a decrease in the megakaryocytic lineage, were observed suggesting a central component of the thrombocytopenia. Bone marrow MK exhibited significantly increased emperipolesis and vacuolization. Moreover, transmission electron microscopy pointed to the presence of viral particles inside bone marrow MK. Immunolabeling confirmed the presence of two SARS-CoV-2 proteins, spike and Orf3a, as well as double-stranded RNA suggesting a potential viral replication cycle.</p><p><strong>Conclusion: </strong>In this series of COVID-19 patients with thrombocytopenia we report the presence of SARS-CoV-2 in bone marrow MK as well as a decrease in MK lineage and an increase in emperipolesis.</p>\",\"PeriodicalId\":17326,\"journal\":{\"name\":\"Journal of Thrombosis and Haemostasis\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2025-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Thrombosis and Haemostasis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jtha.2025.03.036\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Thrombosis and Haemostasis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jtha.2025.03.036","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HEMATOLOGY","Score":null,"Total":0}
Detection of SARS-CoV-2 in bone marrow megakaryocytes and elevated emperipolesis in COVID-19 patients with thrombocytopenia.
Background: Thrombocytopenia and altered platelet activation correlate with COVID-19 severity and mortality. COVID-19 patients have modifications of the platelet and blood circulating megakaryocytes (MK) transcriptome. Our objective was to explore the features of bone marrow MK, which remain poorly characterized in SARS-CoV-2 infected patients, particularly those with thrombocytopenia.
Methods: In this case series study, we analyzed bone marrow samples from a series of 11 COVID-19 patients with thrombocytopenia admitted to the intensive care unit for acute respiratory distress syndrome. Bone marrow sampling, aimed to explore thrombocytopenia's etiology by cytology, allowed us to document bone marrow MK behavior.
Results: A reduction in bone marrow cellularity and a decrease in the megakaryocytic lineage, were observed suggesting a central component of the thrombocytopenia. Bone marrow MK exhibited significantly increased emperipolesis and vacuolization. Moreover, transmission electron microscopy pointed to the presence of viral particles inside bone marrow MK. Immunolabeling confirmed the presence of two SARS-CoV-2 proteins, spike and Orf3a, as well as double-stranded RNA suggesting a potential viral replication cycle.
Conclusion: In this series of COVID-19 patients with thrombocytopenia we report the presence of SARS-CoV-2 in bone marrow MK as well as a decrease in MK lineage and an increase in emperipolesis.
期刊介绍：
The Journal of Thrombosis and Haemostasis (JTH) serves as the official journal of the International Society on Thrombosis and Haemostasis. It is dedicated to advancing science related to thrombosis, bleeding disorders, and vascular biology through the dissemination and exchange of information and ideas within the global research community.
Types of Publications:
The journal publishes a variety of content, including:
Original research reports
State-of-the-art reviews
Brief reports
Case reports
Invited commentaries on publications in the Journal
Forum articles
Correspondence
Announcements
Scope of Contributions:
Editors invite contributions from both fundamental and clinical domains. These include:
Basic manuscripts on blood coagulation and fibrinolysis
Studies on proteins and reactions related to thrombosis and haemostasis
Research on blood platelets and their interactions with other biological systems, such as the vessel wall, blood cells, and invading organisms
Clinical manuscripts covering various topics including venous thrombosis, arterial disease, hemophilia, bleeding disorders, and platelet diseases
Clinical manuscripts may encompass etiology, diagnostics, prognosis, prevention, and treatment strategies.
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