用SANS/FTIR-ATR同时测量观察淀粉退化结构变化动力学。

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of applied glycoscience Pub Date : 2025-02-20 eCollection Date: 2025-01-01 DOI:10.5458/jag.7201102
Yoshinobu Hirata, Fumitoshi Kaneko, Aurel Radulescu, Takahisa Nishizu, Nakako Katsuno, Teppei Imaizumi, Ryuhei Motokawa, Takayuki Kumada, Hiroshi Nakagawa
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引用次数: 0

摘要

由于淀粉具有复杂的层次结构,对淀粉降解的评价通常是综合多种覆盖不同空间尺度的结构分析方法。然而,结构分析通常是单独进行的,这使得在不同空间尺度上关联结构变化具有挑战性。因此,本研究采用由小角中子散射(SANS)/傅立叶变换红外(FTIR)衰减全反射(ATR)组成的同步测量系统来记录马铃薯淀粉在退化过程中的多种结构变化。在SANS模式中,肩部峰值随着时间的推移变得更加明显。峰值强度,imax,代表有序半晶结构的数量,随着时间的推移而增加,揭示了淀粉在纳米级上的有序重组。在FTIR-ATR光谱中,淀粉在1042 cm-1和1016 cm-1处的吸收比(r1042 /1016)随着降解而增加,表明淀粉具有较短的有序结构。因此,在退化过程中,双螺旋结构发生了重组。r1042 /1016的动力学变化速率常数大于imax;因此,淀粉的短程有序结构的变化在半晶结构的变化之前收敛。这些结果表明,支链淀粉侧链的双螺旋结构的形成及其有序排列的结构变化可能在退化过程中分阶段发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetics of Structural Changes in Starch Retrogradation Observed by Simultaneous SANS/FTIR-ATR Measurements.

Because of the complicated hierarchical structure of starch, starch retrogradation is usually evaluated by combining several structural analysis methods covering various spatial scales. However, structural analyses are typically performed individually, making correlating the structural changes at different spatial scales challenging. Therefore, this study used a simultaneous measurement system comprising small-angle neutron scattering (SANS)/Fourier-transform infrared (FTIR)-attenuated total reflection (ATR) to record multiple structural changes in potato starch during retrogradation. In the SANS patterns, the shoulder-like peak became more pronounced with time. The peak intensity, I max, representing the amount of ordered semicrystalline structures, increased over time, revealing the orderly reassembly of starch on the nanoscale upon retrogradation. In the FTIR-ATR spectra, the ratio of absorptions (R 1042/1016) at 1,042 and 1,016 cm-1, indicating the short-range ordered structure in starch, increased during retrogradation. Therefore, the double-helix structures were reformed during retrogradation. The rate constant of the kinetic change for R 1042/1016 was larger than for I max; thus, changes in the short-range ordered structure of starch converged before the changes in the semicrystalline structure. These results suggest that the formation of double-helix structures of the amylopectin side chain and the structural change of its ordered arrangement could occur in stages during retrogradation.

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来源期刊
Journal of applied glycoscience
Journal of applied glycoscience BIOCHEMISTRY & MOLECULAR BIOLOGY-
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