CD147高的经典单核细胞:COVID-19疾病严重程度和治疗反应的细胞生物标记。

Teruaki Murakami, Yuta Yamaguchi, Saori Amiya, Yuko Yoshimine, Shinichiro Nameki, Yasutaka Okita, Yasuhiro Kato, Haruhiko Hirata, Yoshito Takeda, Atsushi Kumanogoh, Takayoshi Morita
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摘要

背景:严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)感染可导致以细胞因子风暴和器官功能障碍为特征的严重冠状病毒病2019 (COVID-19)。刺突S1亚基诱导炎症细胞因子的产生,但免疫细胞亚群响应S1刺激并导致疾病严重程度仍不清楚。方法:分析COVID-19患者的血清和外周血单个核细胞(pbmc)(中度:n = 7;重症患者:n = 25)和健康对照组(n = 38)。使用质量细胞术(飞行时间细胞术;我们分析了健康供体和COVID-19患者外周血单核细胞对S1亚基刺激的免疫细胞反应。我们检查了鉴定的细胞群、血清细胞因子水平和临床参数之间的相关性。结果:血清S1亚基水平与疾病严重程度和炎症细胞因子浓度相关。S1亚基刺激诱导PBMCs产生剂量依赖性细胞因子,主要来自骨髓细胞。CyTOF分析发现CD147高表达的经典单核细胞(CD147hi cMono)是s1诱导的细胞因子的主要来源。CD147hi - cMono的比例在重症COVID-19中显著升高,随着临床改善而降低。与老年患者相比,年轻患者CD147hi cMono的频率与临床严重程度指标的正相关更强。结论:CD147hi cMono是s1诱导的炎症细胞因子的主要细胞来源,可能作为监测COVID-19严重程度和治疗反应的潜在生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CD147-high classical monocytes: a cellular biomarker for COVID-19 disease severity and treatment response.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can lead to severe coronavirus disease 2019 (COVID-19), which is characterized by cytokine storm and organ dysfunction. The spike S1 subunit induces inflammatory cytokine production, but the immune cell subsets that respond to S1 stimulation and contribute to disease severity remain unclear.

Methods: We analyzed serum samples and peripheral blood mononuclear cells (PBMCs) from patients with COVID-19 (moderate: n = 7; severe: n = 25) and healthy controls (n = 38). Using mass cytometry (cytometry by time-of-flight; CyTOF), we analyzed immune cell responses to S1 subunit stimulation in PBMCs from healthy donors and patients with COVID-19. We examined correlations among identified cell populations, serum cytokine levels, and clinical parameters.

Results: Serum S1 subunit levels correlated with disease severity and inflammatory cytokine concentrations. S1 subunit stimulation induced dose-dependent cytokine production from PBMCs, predominantly from myeloid cells. CyTOF analysis identified classical monocytes with high CD147 expression (CD147hi cMono) as the primary source of S1-induced cytokines. The proportion of CD147hi cMono increased significantly in severe COVID-19 and decreased with clinical improvement. The frequency of CD147hi cMono showed a stronger positive correlation with clinical severity markers in younger patients compared to older patients.

Conclusions: CD147hi cMono are the primary cellular source of S1-induced inflammatory cytokines and may serve as potential biomarkers for monitoring COVID-19 severity and treatment response.

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