Teresa Saavedra, Maribela Pestana, José Paulo Da Silva, Pedro José Correia
{"title":"缺铁条件下禾本科植物根系代谢产物的释放","authors":"Teresa Saavedra, Maribela Pestana, José Paulo Da Silva, Pedro José Correia","doi":"10.1002/jpln.202400307","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Strategy-II, as a response mechanism, is limited to grasses under Fe deficiency and is based on the secretion of phytosiderophores (PS) into the rhizosphere, in conjunction with the induction of a high-affinity system for Fe(III)-PS uptake. This strategy has a significant ecological impact and is essential for sustainable agriculture.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>In this work, we studied the physiological and metabolic responses of <i>Poa pratensis</i> L., <i>Lolium perenne</i> L., and <i>Festuca rubra</i> L. when grown in nutrient solution under Fe deficiency.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>After chlorosis appeared, we analyzed leaf pigment concentrations, biomass, and ferric chelate reductase (FCR) activity of root tips. We also screened the metabolites released by root exudates using liquid chromatography coupled to high-resolution mass spectrometry (LC–HRMS).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Plants grown with low or no Fe availability had significantly lower fresh weight (FW) and leaf chlorophyll (Chl) but higher FCR activity as compared to control plants. The root exudates contain carboxylic acids, phenolic acids, polyphenols, and peptides, among others. An increase in carboxylic acid content was correlated with Fe chlorosis in all species studied. However, the specific compounds that were upregulated varied depending on the species. Root exudates contained several upregulated metabolites that were identified as small peptides and/or peptide derivates based on their spectral data and formulas that are compatible with small peptides and/or peptide derivatives.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>These results, together with the metal binding/chelation properties of many peptides, highlight the release of these compounds and/or their derivatives as a response of grasses to increase the Fe bioavailability.</p>\n </section>\n </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"188 2","pages":"312-323"},"PeriodicalIF":2.6000,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolites Released by Poaceae Roots Under Iron-Deficient Conditions\",\"authors\":\"Teresa Saavedra, Maribela Pestana, José Paulo Da Silva, Pedro José Correia\",\"doi\":\"10.1002/jpln.202400307\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Strategy-II, as a response mechanism, is limited to grasses under Fe deficiency and is based on the secretion of phytosiderophores (PS) into the rhizosphere, in conjunction with the induction of a high-affinity system for Fe(III)-PS uptake. This strategy has a significant ecological impact and is essential for sustainable agriculture.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>In this work, we studied the physiological and metabolic responses of <i>Poa pratensis</i> L., <i>Lolium perenne</i> L., and <i>Festuca rubra</i> L. when grown in nutrient solution under Fe deficiency.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>After chlorosis appeared, we analyzed leaf pigment concentrations, biomass, and ferric chelate reductase (FCR) activity of root tips. We also screened the metabolites released by root exudates using liquid chromatography coupled to high-resolution mass spectrometry (LC–HRMS).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Plants grown with low or no Fe availability had significantly lower fresh weight (FW) and leaf chlorophyll (Chl) but higher FCR activity as compared to control plants. The root exudates contain carboxylic acids, phenolic acids, polyphenols, and peptides, among others. An increase in carboxylic acid content was correlated with Fe chlorosis in all species studied. However, the specific compounds that were upregulated varied depending on the species. Root exudates contained several upregulated metabolites that were identified as small peptides and/or peptide derivates based on their spectral data and formulas that are compatible with small peptides and/or peptide derivatives.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>These results, together with the metal binding/chelation properties of many peptides, highlight the release of these compounds and/or their derivatives as a response of grasses to increase the Fe bioavailability.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16802,\"journal\":{\"name\":\"Journal of Plant Nutrition and Soil Science\",\"volume\":\"188 2\",\"pages\":\"312-323\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-02-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Nutrition and Soil Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jpln.202400307\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Nutrition and Soil Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jpln.202400307","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Metabolites Released by Poaceae Roots Under Iron-Deficient Conditions
Background
Strategy-II, as a response mechanism, is limited to grasses under Fe deficiency and is based on the secretion of phytosiderophores (PS) into the rhizosphere, in conjunction with the induction of a high-affinity system for Fe(III)-PS uptake. This strategy has a significant ecological impact and is essential for sustainable agriculture.
Aims
In this work, we studied the physiological and metabolic responses of Poa pratensis L., Lolium perenne L., and Festuca rubra L. when grown in nutrient solution under Fe deficiency.
Methods
After chlorosis appeared, we analyzed leaf pigment concentrations, biomass, and ferric chelate reductase (FCR) activity of root tips. We also screened the metabolites released by root exudates using liquid chromatography coupled to high-resolution mass spectrometry (LC–HRMS).
Results
Plants grown with low or no Fe availability had significantly lower fresh weight (FW) and leaf chlorophyll (Chl) but higher FCR activity as compared to control plants. The root exudates contain carboxylic acids, phenolic acids, polyphenols, and peptides, among others. An increase in carboxylic acid content was correlated with Fe chlorosis in all species studied. However, the specific compounds that were upregulated varied depending on the species. Root exudates contained several upregulated metabolites that were identified as small peptides and/or peptide derivates based on their spectral data and formulas that are compatible with small peptides and/or peptide derivatives.
Conclusions
These results, together with the metal binding/chelation properties of many peptides, highlight the release of these compounds and/or their derivatives as a response of grasses to increase the Fe bioavailability.
期刊介绍:
Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years.
Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH.
Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are:
JPNSS – Topical Divisions
Special timely focus in interdisciplinarity:
- sustainability & critical zone science.
Soil-Plant Interactions:
- rhizosphere science & soil ecology
- pollutant cycling & plant-soil protection
- land use & climate change.
Soil Science:
- soil chemistry & soil physics
- soil biology & biogeochemistry
- soil genesis & mineralogy.
Plant Nutrition:
- plant nutritional physiology
- nutrient dynamics & soil fertility
- ecophysiological aspects of plant nutrition.