纳米光盘仍是膜蛋白冷冻电镜研究不可或缺的工具

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-04-08 DOI:10.1016/j.sbi.2025.103042

Giorgos Hiotis , Ryan Q. Notti , Huan Bao , Thomas Walz

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引用次数: 0

摘要

纳米片是由膜支架蛋白（MSPs）稳定的小盘状膜斑块，是稳定整体膜蛋白（imp）的常用工具，用于低温电子显微镜（cro - em）的结构研究。虽然纳米圆盘为整合的imp提供了接近天然的膜环境，但它们并不能再现天然膜的所有特征。此外，imp必须首先在洗涤剂中纯化，然后才能重组为基于msp的纳米圆盘，这一问题已经被新的方法所克服，例如基于共聚物的天然纳米圆盘和细胞衍生的囊泡。在这篇综述中，我们认为尽管取得了这些进展，基于msp的纳米片仍然是研究imp结构的独特工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nanodiscs remain indispensable for Cryo-EM studies of membrane proteins

Nanodiscs, small discoidal membrane patches stabilized by membrane-scaffold proteins (MSPs), are popular tools to stabilize integral membrane proteins (IMPs) for structural studies by cryogenic electron microscopy (cryo-EM). While nanodiscs provide a near-native membrane environment for the incorporated IMPs, they do not reproduce all characteristics of a native membrane. Also, IMPs must first be purified in detergent before they can be reconstituted into MSP-based nanodiscs, a problem that has been overcome by newer approaches, such as copolymer-based native nanodiscs and cell-derived vesicles. In this review, we argue that despite these advances, MSP-based nanodiscs remain a unique tool for the structural interrogation of IMPs.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation