{"title":"超声波蒸汽提取牛油叶生物活性化合物：生物活性、抗菌及LC-MS /MS研究","authors":"Salam Maheshkumar Singh, Soubhagya Tripathy, Prem Prakash Srivastav","doi":"10.1007/s10068-024-01810-x","DOIUrl":null,"url":null,"abstract":"<div><p>Giloy (<i>Tinospora cordifolia</i>) is a medicinal plant rich in bioactive compounds known for their diverse health benefits. This study examined the nutritional value and biological activity of giloy stems and leaves. Hence, giloy stem (GSE), and leaf extract (GLE) was extracted using green extraction technology, ultrasound, and screening secondary metabolites and bioactive compounds. Using a central composite rotatable design combined with RSM, GSE, and GLE's antioxidant activity, total phenolic content and yield were optimized for solvent-to-solid ratio, sonication time, and solvent concertation. The optimum extraction conditions were found to be 22.5:1, 40 min, and 75%. Additionally, the extract inhibited the growth of <i>S. aureus</i> and <i>E. coli</i>. Screening of optimized extract through LC/MS reported the presence of significant polyphenols. Bioactive substances such as catechin, malic acid, quercetin, kaempferol, ellagic acid, hesperidin, and berberine were found. These findings indicate that ultrasonication, a green extraction method, promotes GSE and GLE bioactive chemical extraction. Giloy can make tasty, long-lasting food and drinks as a functional ingredient.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 9","pages":"1835 - 1847"},"PeriodicalIF":2.4000,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioactive compound extraction from giloy leaves and steam using ultrasound: bioactivity, antimicrobial, and LC–MS/MS study\",\"authors\":\"Salam Maheshkumar Singh, Soubhagya Tripathy, Prem Prakash Srivastav\",\"doi\":\"10.1007/s10068-024-01810-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Giloy (<i>Tinospora cordifolia</i>) is a medicinal plant rich in bioactive compounds known for their diverse health benefits. This study examined the nutritional value and biological activity of giloy stems and leaves. Hence, giloy stem (GSE), and leaf extract (GLE) was extracted using green extraction technology, ultrasound, and screening secondary metabolites and bioactive compounds. Using a central composite rotatable design combined with RSM, GSE, and GLE's antioxidant activity, total phenolic content and yield were optimized for solvent-to-solid ratio, sonication time, and solvent concertation. The optimum extraction conditions were found to be 22.5:1, 40 min, and 75%. Additionally, the extract inhibited the growth of <i>S. aureus</i> and <i>E. coli</i>. Screening of optimized extract through LC/MS reported the presence of significant polyphenols. Bioactive substances such as catechin, malic acid, quercetin, kaempferol, ellagic acid, hesperidin, and berberine were found. These findings indicate that ultrasonication, a green extraction method, promotes GSE and GLE bioactive chemical extraction. Giloy can make tasty, long-lasting food and drinks as a functional ingredient.</p></div>\",\"PeriodicalId\":566,\"journal\":{\"name\":\"Food Science and Biotechnology\",\"volume\":\"34 9\",\"pages\":\"1835 - 1847\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Science and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10068-024-01810-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Science and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10068-024-01810-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Bioactive compound extraction from giloy leaves and steam using ultrasound: bioactivity, antimicrobial, and LC–MS/MS study
Giloy (Tinospora cordifolia) is a medicinal plant rich in bioactive compounds known for their diverse health benefits. This study examined the nutritional value and biological activity of giloy stems and leaves. Hence, giloy stem (GSE), and leaf extract (GLE) was extracted using green extraction technology, ultrasound, and screening secondary metabolites and bioactive compounds. Using a central composite rotatable design combined with RSM, GSE, and GLE's antioxidant activity, total phenolic content and yield were optimized for solvent-to-solid ratio, sonication time, and solvent concertation. The optimum extraction conditions were found to be 22.5:1, 40 min, and 75%. Additionally, the extract inhibited the growth of S. aureus and E. coli. Screening of optimized extract through LC/MS reported the presence of significant polyphenols. Bioactive substances such as catechin, malic acid, quercetin, kaempferol, ellagic acid, hesperidin, and berberine were found. These findings indicate that ultrasonication, a green extraction method, promotes GSE and GLE bioactive chemical extraction. Giloy can make tasty, long-lasting food and drinks as a functional ingredient.
期刊介绍：
The FSB journal covers food chemistry and analysis for compositional and physiological activity changes, food hygiene and toxicology, food microbiology and biotechnology, and food engineering involved in during and after food processing through physical, chemical, and biological ways. Consumer perception and sensory evaluation on processed foods are accepted only when they are relevant to the laboratory research work. As a general rule, manuscripts dealing with analysis and efficacy of extracts from natural resources prior to the processing or without any related food processing may not be considered within the scope of the journal. The FSB journal does not deal with only local interest and a lack of significant scientific merit. The main scope of our journal is seeking for human health and wellness through constructive works and new findings in food science and biotechnology field.
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