{"title":"PBMC衍生lncRNAs在类风湿关节炎中的表达及意义","authors":"Xiaoke Yang, Zhongling Yang, Ziqiang Shuai, Mingming Zhang, Sheng-Qian Xu, Zong Wen Shuai","doi":"10.3389/fimmu.2025.1515665","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Long non-coding RNAs (lncRNAs) are gaining recognition for their critical involvement in diverse autoimmune disorders. Nevertheless, reseach investigating the role of lncRNAs in rheumatoid arthritis (RA) is relatively scarce.</p><p><strong>Methods: </strong>Comprehensive transcriptome sequencing was executed to acquire a lncRNA expression pattern in peripheral blood mononuclear cells (PBMC) of RA. Then, we confirmed the sequencing data by real-time quantitative polymerase chain reaction (RT-qPCR).</p><p><strong>Results: </strong>The findings showed decreased levels of LINC00494, TSP0AP1-AS1, MCM3AP-AS1 and LINC01588, increased levels of OIP5-AS1, in PBMC of RA compared to controls. ROC analysis for the five dysregulated lncRNAs demonstrated an area under curve (AUC) extending from 0.654 to 0.915, and their combination had high utility for accurate RA diagnosis (AUC = 0.920). There existed a negative relation between RF and LINC00494 expression (<i>P</i>=0.027), positive relation between anti-CCP and MCM3AP-AS1 (<i>P</i>=0.024), and negative relation between CRP and LINC01588 expression (<i>P</i>=0.020).</p><p><strong>Conclusions: </strong>Our study indicated that LINC00494, TSP0AP1-AS1, MCM3AP-AS1, LINC01588 and OIP5-AS1 in PBMC may be the biomarkers for RA.</p>","PeriodicalId":12622,"journal":{"name":"Frontiers in Immunology","volume":"16 ","pages":"1515665"},"PeriodicalIF":5.7000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965121/pdf/","citationCount":"0","resultStr":"{\"title\":\"Expression and significance of lncRNAs derived from PBMC in rheumatoid arthritis.\",\"authors\":\"Xiaoke Yang, Zhongling Yang, Ziqiang Shuai, Mingming Zhang, Sheng-Qian Xu, Zong Wen Shuai\",\"doi\":\"10.3389/fimmu.2025.1515665\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Long non-coding RNAs (lncRNAs) are gaining recognition for their critical involvement in diverse autoimmune disorders. Nevertheless, reseach investigating the role of lncRNAs in rheumatoid arthritis (RA) is relatively scarce.</p><p><strong>Methods: </strong>Comprehensive transcriptome sequencing was executed to acquire a lncRNA expression pattern in peripheral blood mononuclear cells (PBMC) of RA. Then, we confirmed the sequencing data by real-time quantitative polymerase chain reaction (RT-qPCR).</p><p><strong>Results: </strong>The findings showed decreased levels of LINC00494, TSP0AP1-AS1, MCM3AP-AS1 and LINC01588, increased levels of OIP5-AS1, in PBMC of RA compared to controls. ROC analysis for the five dysregulated lncRNAs demonstrated an area under curve (AUC) extending from 0.654 to 0.915, and their combination had high utility for accurate RA diagnosis (AUC = 0.920). There existed a negative relation between RF and LINC00494 expression (<i>P</i>=0.027), positive relation between anti-CCP and MCM3AP-AS1 (<i>P</i>=0.024), and negative relation between CRP and LINC01588 expression (<i>P</i>=0.020).</p><p><strong>Conclusions: </strong>Our study indicated that LINC00494, TSP0AP1-AS1, MCM3AP-AS1, LINC01588 and OIP5-AS1 in PBMC may be the biomarkers for RA.</p>\",\"PeriodicalId\":12622,\"journal\":{\"name\":\"Frontiers in Immunology\",\"volume\":\"16 \",\"pages\":\"1515665\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965121/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fimmu.2025.1515665\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fimmu.2025.1515665","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Expression and significance of lncRNAs derived from PBMC in rheumatoid arthritis.
Background: Long non-coding RNAs (lncRNAs) are gaining recognition for their critical involvement in diverse autoimmune disorders. Nevertheless, reseach investigating the role of lncRNAs in rheumatoid arthritis (RA) is relatively scarce.
Methods: Comprehensive transcriptome sequencing was executed to acquire a lncRNA expression pattern in peripheral blood mononuclear cells (PBMC) of RA. Then, we confirmed the sequencing data by real-time quantitative polymerase chain reaction (RT-qPCR).
Results: The findings showed decreased levels of LINC00494, TSP0AP1-AS1, MCM3AP-AS1 and LINC01588, increased levels of OIP5-AS1, in PBMC of RA compared to controls. ROC analysis for the five dysregulated lncRNAs demonstrated an area under curve (AUC) extending from 0.654 to 0.915, and their combination had high utility for accurate RA diagnosis (AUC = 0.920). There existed a negative relation between RF and LINC00494 expression (P=0.027), positive relation between anti-CCP and MCM3AP-AS1 (P=0.024), and negative relation between CRP and LINC01588 expression (P=0.020).
Conclusions: Our study indicated that LINC00494, TSP0AP1-AS1, MCM3AP-AS1, LINC01588 and OIP5-AS1 in PBMC may be the biomarkers for RA.
期刊介绍：
Frontiers in Immunology is a leading journal in its field, publishing rigorously peer-reviewed research across basic, translational and clinical immunology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Immunology is the official Journal of the International Union of Immunological Societies (IUIS). Encompassing the entire field of Immunology, this journal welcomes papers that investigate basic mechanisms of immune system development and function, with a particular emphasis given to the description of the clinical and immunological phenotype of human immune disorders, and on the definition of their molecular basis.
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