Yichen Gao, Xianyi He, Yuyao Deng, Xia Wang, Jie Shen, Wei Xu, Rui Cai, Xiaorong Lan, Junliang Chen, Yun He
{"title":"人牙滤泡细胞来源的条件培养基通过调节牙周韧带干细胞的成骨分化和炎症以及巨噬细胞极化来促进牙周再生。","authors":"Yichen Gao, Xianyi He, Yuyao Deng, Xia Wang, Jie Shen, Wei Xu, Rui Cai, Xiaorong Lan, Junliang Chen, Yun He","doi":"10.1007/s11010-025-05260-9","DOIUrl":null,"url":null,"abstract":"<p><p>Dental follicle cells (DFCs) derived from the neural crest are promising seed cells for periodontal tissue engineering. This study aimed to investigate whether conditioned media (CM) from human DFCs could regenerate impaired periodontal tissue and the underlying mechanisms. hDFC-derived CM (hDFC-CM) were obtained via ultracentrifugation. In vitro, human periodontal ligament stem cells (hPDLSCs) were treated with hDFC-CM in normal and inflammatory microenvironments, and the cell proliferation, migration, and osteogenic potential were evaluated. We simulated the inflammatory environment with lipopolysaccharide and detected the expression of osteogenic and Wnt/β-catenin signaling pathway-related proteins. The effect of hDFC-CM on the inhibition of hPDLSC inflammation and macrophage polarization was examined. In vivo, the rat periodontitis model was treated with hDFC-CM. Tissue samples were collected after 4 weeks for micro-computed tomography and histological examination. The results of cell counting kit-8 and scratch experiments showed that hDFC-CM significantly enhanced the proliferation and migration capacities of hPDLSCs. hDFC-CM promoted the osteogenic differentiation of hPDLSCs by showing intense alkaline phosphatase and Alizarin Red staining and upregulated osteogenic protein and gene expression. Western blotting also verified that hDFC-CM promotes the osteogenic differentiation of hPDLSCs by regulating the Wnt/β-catenin pathway in an inflammatory environment. In addition, hDFC-CM inhibited hPDLSC inflammation and polarized macrophages from the M1 to M2 phenotype. In vivo, hDFC-CM effectively promoted periodontal tissue regeneration. hDFC-CM enhances periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization, which provided new biochemical cues for the treatment of periodontitis.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":"4431-4448"},"PeriodicalIF":3.5000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Human dental follicle cell-derived conditioned media enhance periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization.\",\"authors\":\"Yichen Gao, Xianyi He, Yuyao Deng, Xia Wang, Jie Shen, Wei Xu, Rui Cai, Xiaorong Lan, Junliang Chen, Yun He\",\"doi\":\"10.1007/s11010-025-05260-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Dental follicle cells (DFCs) derived from the neural crest are promising seed cells for periodontal tissue engineering. This study aimed to investigate whether conditioned media (CM) from human DFCs could regenerate impaired periodontal tissue and the underlying mechanisms. hDFC-derived CM (hDFC-CM) were obtained via ultracentrifugation. In vitro, human periodontal ligament stem cells (hPDLSCs) were treated with hDFC-CM in normal and inflammatory microenvironments, and the cell proliferation, migration, and osteogenic potential were evaluated. We simulated the inflammatory environment with lipopolysaccharide and detected the expression of osteogenic and Wnt/β-catenin signaling pathway-related proteins. The effect of hDFC-CM on the inhibition of hPDLSC inflammation and macrophage polarization was examined. In vivo, the rat periodontitis model was treated with hDFC-CM. Tissue samples were collected after 4 weeks for micro-computed tomography and histological examination. The results of cell counting kit-8 and scratch experiments showed that hDFC-CM significantly enhanced the proliferation and migration capacities of hPDLSCs. hDFC-CM promoted the osteogenic differentiation of hPDLSCs by showing intense alkaline phosphatase and Alizarin Red staining and upregulated osteogenic protein and gene expression. Western blotting also verified that hDFC-CM promotes the osteogenic differentiation of hPDLSCs by regulating the Wnt/β-catenin pathway in an inflammatory environment. In addition, hDFC-CM inhibited hPDLSC inflammation and polarized macrophages from the M1 to M2 phenotype. In vivo, hDFC-CM effectively promoted periodontal tissue regeneration. hDFC-CM enhances periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization, which provided new biochemical cues for the treatment of periodontitis.</p>\",\"PeriodicalId\":18724,\"journal\":{\"name\":\"Molecular and Cellular Biochemistry\",\"volume\":\" \",\"pages\":\"4431-4448\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and Cellular Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11010-025-05260-9\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11010-025-05260-9","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/2 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Human dental follicle cell-derived conditioned media enhance periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization.
Dental follicle cells (DFCs) derived from the neural crest are promising seed cells for periodontal tissue engineering. This study aimed to investigate whether conditioned media (CM) from human DFCs could regenerate impaired periodontal tissue and the underlying mechanisms. hDFC-derived CM (hDFC-CM) were obtained via ultracentrifugation. In vitro, human periodontal ligament stem cells (hPDLSCs) were treated with hDFC-CM in normal and inflammatory microenvironments, and the cell proliferation, migration, and osteogenic potential were evaluated. We simulated the inflammatory environment with lipopolysaccharide and detected the expression of osteogenic and Wnt/β-catenin signaling pathway-related proteins. The effect of hDFC-CM on the inhibition of hPDLSC inflammation and macrophage polarization was examined. In vivo, the rat periodontitis model was treated with hDFC-CM. Tissue samples were collected after 4 weeks for micro-computed tomography and histological examination. The results of cell counting kit-8 and scratch experiments showed that hDFC-CM significantly enhanced the proliferation and migration capacities of hPDLSCs. hDFC-CM promoted the osteogenic differentiation of hPDLSCs by showing intense alkaline phosphatase and Alizarin Red staining and upregulated osteogenic protein and gene expression. Western blotting also verified that hDFC-CM promotes the osteogenic differentiation of hPDLSCs by regulating the Wnt/β-catenin pathway in an inflammatory environment. In addition, hDFC-CM inhibited hPDLSC inflammation and polarized macrophages from the M1 to M2 phenotype. In vivo, hDFC-CM effectively promoted periodontal tissue regeneration. hDFC-CM enhances periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization, which provided new biochemical cues for the treatment of periodontitis.
期刊介绍：
Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell.
In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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