Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu
{"title":"运动通过Janus激酶2/信号转导因子和转录3信号通路激活因子调控自发性高血压的机制","authors":"Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu","doi":"10.4103/ejpi.EJPI-D-24-00085","DOIUrl":null,"url":null,"abstract":"<p><strong>Abstract: </strong>Although blood pressure can be regulated to normal levels through drug intervention, the prognosis for patients remains bleak, and there is an urgent need to find a new way to prevent or reverse hypertension and its adverse consequences. Some studies have shown that hypertension can be effectively improved by exercise training, but the exact manner in which it works is not completely comprehended. Using spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats as models, an 8-week exercise intervention was adopted to detect the blood pressure, heart rate (HR), glucose tolerance, and insulin sensitivity. Histopathological changes in rat myocardium were observed using H and E staining and Masson staining. Different kits were used to detect the inflammation and oxidative stress in rats. Finally, the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway-related proteins were examined by Western blot. After exercise, SHR rats showed a decrease in blood pressure and HR level, and an increase in glucose tolerance and myocardial insulin sensitivity. Exercise reduced SHR cardiomyocyte cross-sectional area and collagen deposition index, improved cardiac function, and decreased inflammation and oxidative stress. In addition, exercise can modulate the JAK2/STAT3 pathway, and when combined with JAK2 kinase inhibitor, it has an even greater effect on SHR. In conclusion, exercise could reduce blood pressure, inflammation, and oxidative stress, improve glucose tolerance and insulin sensitivity in SHR rats through modulating the JAK2/STAT3 pathway.</p>","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":" ","pages":"109-119"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanism of Action of Exercise in Regulating Spontaneous Hypertension through the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling Pathway.\",\"authors\":\"Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu\",\"doi\":\"10.4103/ejpi.EJPI-D-24-00085\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Abstract: </strong>Although blood pressure can be regulated to normal levels through drug intervention, the prognosis for patients remains bleak, and there is an urgent need to find a new way to prevent or reverse hypertension and its adverse consequences. Some studies have shown that hypertension can be effectively improved by exercise training, but the exact manner in which it works is not completely comprehended. Using spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats as models, an 8-week exercise intervention was adopted to detect the blood pressure, heart rate (HR), glucose tolerance, and insulin sensitivity. Histopathological changes in rat myocardium were observed using H and E staining and Masson staining. Different kits were used to detect the inflammation and oxidative stress in rats. Finally, the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway-related proteins were examined by Western blot. After exercise, SHR rats showed a decrease in blood pressure and HR level, and an increase in glucose tolerance and myocardial insulin sensitivity. Exercise reduced SHR cardiomyocyte cross-sectional area and collagen deposition index, improved cardiac function, and decreased inflammation and oxidative stress. In addition, exercise can modulate the JAK2/STAT3 pathway, and when combined with JAK2 kinase inhibitor, it has an even greater effect on SHR. In conclusion, exercise could reduce blood pressure, inflammation, and oxidative stress, improve glucose tolerance and insulin sensitivity in SHR rats through modulating the JAK2/STAT3 pathway.</p>\",\"PeriodicalId\":519921,\"journal\":{\"name\":\"Journal of physiological investigation\",\"volume\":\" \",\"pages\":\"109-119\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of physiological investigation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/ejpi.EJPI-D-24-00085\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/31 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of physiological investigation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ejpi.EJPI-D-24-00085","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/31 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
摘要:虽然通过药物干预可以将血压调节到正常水平,但患者预后仍不容乐观,迫切需要寻找预防或逆转高血压及其不良后果的新途径。一些研究表明,通过运动训练可以有效地改善高血压,但其工作的确切方式尚不完全清楚。以自发性高血压大鼠(SHR)和Wistar-Kyoto大鼠为模型,采用运动干预8周的方法检测血压、心率(HR)、葡萄糖耐量和胰岛素敏感性。采用H、E染色和Masson染色观察大鼠心肌组织病理学变化。采用不同的试剂盒检测大鼠的炎症和氧化应激。最后,采用Western blot检测Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3)通路相关蛋白。运动后,SHR大鼠血压和HR水平下降,葡萄糖耐量和心肌胰岛素敏感性升高。运动可降低SHR心肌细胞横截面积和胶原沉积指数,改善心功能,减轻炎症和氧化应激。此外,运动可以调节JAK2/STAT3通路,当与JAK2激酶抑制剂联合使用时,对SHR的影响更大。综上所述,运动可以通过调节JAK2/STAT3通路,降低SHR大鼠的血压、炎症和氧化应激,改善糖耐量和胰岛素敏感性。
Mechanism of Action of Exercise in Regulating Spontaneous Hypertension through the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling Pathway.
Abstract: Although blood pressure can be regulated to normal levels through drug intervention, the prognosis for patients remains bleak, and there is an urgent need to find a new way to prevent or reverse hypertension and its adverse consequences. Some studies have shown that hypertension can be effectively improved by exercise training, but the exact manner in which it works is not completely comprehended. Using spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats as models, an 8-week exercise intervention was adopted to detect the blood pressure, heart rate (HR), glucose tolerance, and insulin sensitivity. Histopathological changes in rat myocardium were observed using H and E staining and Masson staining. Different kits were used to detect the inflammation and oxidative stress in rats. Finally, the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway-related proteins were examined by Western blot. After exercise, SHR rats showed a decrease in blood pressure and HR level, and an increase in glucose tolerance and myocardial insulin sensitivity. Exercise reduced SHR cardiomyocyte cross-sectional area and collagen deposition index, improved cardiac function, and decreased inflammation and oxidative stress. In addition, exercise can modulate the JAK2/STAT3 pathway, and when combined with JAK2 kinase inhibitor, it has an even greater effect on SHR. In conclusion, exercise could reduce blood pressure, inflammation, and oxidative stress, improve glucose tolerance and insulin sensitivity in SHR rats through modulating the JAK2/STAT3 pathway.