建立了一种hplc -(ESI)MS/MS法测定圣佩德罗(Trichocereus spp.)致幻次级代谢物美斯卡灵的含量及其在其他仙人掌品种中筛选美斯卡灵的适用性。

IF 2.6 3区 医学 Q2 CHEMISTRY, ANALYTICAL
Pragna Gaur , Liam Engel , Damian Robert Hall , Cheang Khoo , Jerome Sarris , Daniel Perkins , Chun Guang Li , Mitchell Low
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引用次数: 0

摘要

迷幻疗法治疗顽固性精神疾病的临床试验开始后,含有迷幻物质的植物引起了公众越来越多的关注。虽然精神活性生物碱麦司卡林的临床试验有限,但圣佩德罗仙人掌(Trichocereus spp.)在一些司法管辖区,传统使用仍然是合法的,因为监管下的迷幻剂剂量通常被认为是可以承受的,只有轻微中毒的报告,很少有持续的负面心理影响。不过,观赏圣佩德罗中的麦司卡林浓度变化很大,如果被误导用于非法用途,就有可能无意中摄入过少或过多的剂量。本文报告了一种经过验证的超高效液相色谱-(ESI)质谱/质谱(UHPLC-(ESI)MS/MS)方法,用于方便、快速地定量检测圣佩德罗中的麦司卡林,并筛选 12 种可能未经检测的仙人掌。初步结果表明,圣佩德罗仙人掌软骨中的麦司卡林含量可能高于实质中的含量。虽然有六种仙人掌无法检测到麦司卡林,但我们的筛选工作首次对两个品种的 Echinopsis subdenudata 中的麦司卡林进行了定量,含量从 36 μg g-1 到 2.45 mg g-1。从化学分类学的角度来看,在一个传统的棘属物种中发现如此多的麦角碱,进一步表明三色堇属并不独特,并可能支持将它们合并为一个属的争议。从法医学角度来看,鉴定出一种以前不知道含有麦司卡林的流行观赏仙人掌表明,法医学需要对其他仙人掌进行例行筛查,以保持对迷幻药使用新趋势的领先地位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A UHPLC-(ESI)MS/MS method for the determination of the psychedelic secondary metabolite mescaline in San Pedro (Trichocereus spp.) and its applicability for screening mescaline in other cacti varieties.

A UHPLC-(ESI)MS/MS method for the determination of the psychedelic secondary metabolite mescaline in San Pedro (Trichocereus spp.) and its applicability for screening mescaline in other cacti varieties.
Commencements of clinical trials of psychedelic therapies for intractable forms of mental illnesses have drawn increased public attention to plants containing psychedelic substances. Whilst the psychoactive alkaloid mescaline has limited clinical trials, the San Pedro (Trichocereus spp.) cacti from which it is found have a long history of Indigenous medical and spiritual use. Traditional use remains licit in some jurisdictions, as supervised psychedelic dosage is typically regarded as tolerable with reports of only mild intoxications and few persisting negative psychological effects. However, mescaline concentration in ornamental San Pedro is highly variable, introducing risk of unintentional low or high dosage if misdirected for illicit use. This paper reports a validated UHPLC-(ESI)MS/MS method for the convenient and rapid quantification of mescaline in San Pedro, and for screening twelve potentially untested cacti. Preliminary results indicated that there may be higher amounts of mescaline in the chlorenchyma than the parenchyma of San Pedro. While six cacti lacked detectable mescaline, our screening enabled the first quantifications of mescaline in two varieties of Echinopsis subdenudata from 36 μg g−1 to 2.45 mg g−1. Chemotaxonomically, this amount of mescaline in a species from the traditional Echinopsis genus further suggests that the Trichocereus genus is not distinct and may support their contested combination into a single genus. Forensically, the identification of a popular ornamental cactus that was previously not known to contain mescaline demonstrates the need for routine screening of other cacti for the forensic sciences to remain ahead of emerging trends in psychedelic drug use.
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来源期刊
Forensic Chemistry
Forensic Chemistry CHEMISTRY, ANALYTICAL-
CiteScore
5.70
自引率
14.80%
发文量
65
审稿时长
46 days
期刊介绍: Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.
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