Reyes Nicolás de Blas, Miriam Menacho Román, Sara Olivares Romero, Claudia Mesas Mariñán, Alba Arroyo Vega, Graciela Martín Gómez, María Álvarez Pastor, Lucía Castillo Menéndez, María José Azofra Villa, María Del Pilar Pérez Sobrino, Ignacio Arribas Gómez
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Two serum-separating tubes were collected from each patient (Becton Dickinson (BD) Vacutainer<sup>®</sup> SST™ II Advance, 8.5 mL Ref. 366468)<i>.</i> One of the tubes was centrifuged at 2,530 × <i>g</i> for 10 min (control method), while the other tube was centrifuged under alternative centrifugation conditions, namely 2,530 × <i>g</i> for 7 min.</p><p><strong>Results: </strong>The analysis of results revealed that calcium, total bilirubin, and magnesium exhibited a constant and proportional systematic bias. However, considering the proportional mean difference, all analytes met the desirable bias performance established by our laboratory, except for magnesium, which met the minimum bias criteria.</p><p><strong>Conclusions: </strong>Our study demonstrates that both centrifugation protocols are interchangeable for measuring the studied analytes, thereby ensuring adequate sample quality.</p>","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"6 1","pages":"108-112"},"PeriodicalIF":1.1000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949531/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of an alternative centrifugation protocol for reducing total turnaround time.\",\"authors\":\"Reyes Nicolás de Blas, Miriam Menacho Román, Sara Olivares Romero, Claudia Mesas Mariñán, Alba Arroyo Vega, Graciela Martín Gómez, María Álvarez Pastor, Lucía Castillo Menéndez, María José Azofra Villa, María Del Pilar Pérez Sobrino, Ignacio Arribas Gómez\",\"doi\":\"10.1515/almed-2024-0170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Centrifugation is a key process that should be controlled to ensure an adequate sample quality. To achieve rapid, standardized, and consistent sample centrifugation, we aimed to evaluate an alternative centrifugation protocol and its impact on the results of 20 biochemical determinations in serum.</p><p><strong>Methods: </strong>The study included 45 ambulatory patients. Two serum-separating tubes were collected from each patient (Becton Dickinson (BD) Vacutainer<sup>®</sup> SST™ II Advance, 8.5 mL Ref. 366468)<i>.</i> One of the tubes was centrifuged at 2,530 × <i>g</i> for 10 min (control method), while the other tube was centrifuged under alternative centrifugation conditions, namely 2,530 × <i>g</i> for 7 min.</p><p><strong>Results: </strong>The analysis of results revealed that calcium, total bilirubin, and magnesium exhibited a constant and proportional systematic bias. However, considering the proportional mean difference, all analytes met the desirable bias performance established by our laboratory, except for magnesium, which met the minimum bias criteria.</p><p><strong>Conclusions: </strong>Our study demonstrates that both centrifugation protocols are interchangeable for measuring the studied analytes, thereby ensuring adequate sample quality.</p>\",\"PeriodicalId\":72097,\"journal\":{\"name\":\"Advances in laboratory medicine\",\"volume\":\"6 1\",\"pages\":\"108-112\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-11-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949531/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in laboratory medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/almed-2024-0170\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in laboratory medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/almed-2024-0170","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的:离心是一个关键过程,应加以控制以确保足够的样本质量。为了实现快速、标准化和一致的样本离心,我们旨在评估一种替代离心方案及其对 20 项血清生化测定结果的影响:研究对象包括 45 名门诊患者。每位患者采集两支血清分离管(Becton Dickinson (BD) Vacutainer® SST™ II Advance, 8.5 mL Ref.366468).其中一管在 2,530 × g 下离心 10 分钟(对照方法),另一管在其他离心条件下离心,即 2,530 × g 下离心 7 分钟:结果分析表明,钙、总胆红素和镁的系统偏差恒定且成正比。然而,考虑到比例平均差,除了镁达到了最小偏差标准外,其他分析物都达到了本实验室规定的理想偏差性能:我们的研究表明,两种离心方案在测量所研究的分析物时可以互换,从而确保了足够的样本质量。
Evaluation of an alternative centrifugation protocol for reducing total turnaround time.
Objectives: Centrifugation is a key process that should be controlled to ensure an adequate sample quality. To achieve rapid, standardized, and consistent sample centrifugation, we aimed to evaluate an alternative centrifugation protocol and its impact on the results of 20 biochemical determinations in serum.
Methods: The study included 45 ambulatory patients. Two serum-separating tubes were collected from each patient (Becton Dickinson (BD) Vacutainer® SST™ II Advance, 8.5 mL Ref. 366468). One of the tubes was centrifuged at 2,530 × g for 10 min (control method), while the other tube was centrifuged under alternative centrifugation conditions, namely 2,530 × g for 7 min.
Results: The analysis of results revealed that calcium, total bilirubin, and magnesium exhibited a constant and proportional systematic bias. However, considering the proportional mean difference, all analytes met the desirable bias performance established by our laboratory, except for magnesium, which met the minimum bias criteria.
Conclusions: Our study demonstrates that both centrifugation protocols are interchangeable for measuring the studied analytes, thereby ensuring adequate sample quality.