Piotr Adamczuk, Konrad Jamka, Hubert Bojar, Joanna Szala-Rycaj, Aleksandra Szewczyk, Krzysztof Bogumił Sawicki, Grzegorz Raszewski
{"title":"抗氧化剂n -乙酰半胱氨酸对阿托品单用和阿托品与奥比肟联用甲基吡米磷中毒过程中脑和血胆碱酯酶活性的影响","authors":"Piotr Adamczuk, Konrad Jamka, Hubert Bojar, Joanna Szala-Rycaj, Aleksandra Szewczyk, Krzysztof Bogumił Sawicki, Grzegorz Raszewski","doi":"10.26444/aaem/199716","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction and objective: </strong>The antioxidant N-acetylcysteine (NAC) may help in the treatment of organophosphates poisoning, including Pirymiphos methyl (PM). However, there is no information on the effect of NAC on target cholinesterases during the core treatment with atropine and obidoxime after acute and chronic exposure to PM. The impact was investigated of NAC on the functional status of target cholinesterases in the brain and blood during treatment with atropine (ATR) and/or obidoxime (OBID) in PM-induced toxicity.</p><p><strong>Material and methods: </strong>All experiments were performed on Male Swiss mice. The animals were intoxicated with PM and treated with OBID and/or ATR with or without and NAC, in various combinations (with 2-3 drugs) used simultaneously after intoxication. Total acetylcholinesterase activity (AChE) in brain and blood and plasma butyrylcholinesterase activity (BChE) were monitored at 2 and 72 h after intoxication. Enzyme activity was determined using Ellman's colorimetric method.</p><p><strong>Results: </strong>The applied therapies with OBID, ATR and NAC in various configurations significantly reactivated PM-inhibited AChE in the brain and erythrocytes and the BChE in the plasma. The benefits of NAC administration in combination with ATR and/or OBID therapy have also been reported to restore AChE activity in the brain. NAC may reduce the dose of ATR in the treatment of PM poisoning.</p><p><strong>Conclusions: </strong>Adjunctive treatment offered by NAC can reduce or prevent the deleterious effects against PM-induced toxicity. Therefore, NAC remains a strong candidate for adjunct treatment for OP-poisoning, including PM, although additional preclinical and clinical studies are needed.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":"32 1","pages":"116-121"},"PeriodicalIF":1.3000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of the antioxidant N-acetylcysteine on cholinesterase activity in the brain and blood during Pirimiphos methyl poisoning in the course of treatment with atropine alone, and with atropine and obidoxime.\",\"authors\":\"Piotr Adamczuk, Konrad Jamka, Hubert Bojar, Joanna Szala-Rycaj, Aleksandra Szewczyk, Krzysztof Bogumił Sawicki, Grzegorz Raszewski\",\"doi\":\"10.26444/aaem/199716\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction and objective: </strong>The antioxidant N-acetylcysteine (NAC) may help in the treatment of organophosphates poisoning, including Pirymiphos methyl (PM). However, there is no information on the effect of NAC on target cholinesterases during the core treatment with atropine and obidoxime after acute and chronic exposure to PM. The impact was investigated of NAC on the functional status of target cholinesterases in the brain and blood during treatment with atropine (ATR) and/or obidoxime (OBID) in PM-induced toxicity.</p><p><strong>Material and methods: </strong>All experiments were performed on Male Swiss mice. The animals were intoxicated with PM and treated with OBID and/or ATR with or without and NAC, in various combinations (with 2-3 drugs) used simultaneously after intoxication. Total acetylcholinesterase activity (AChE) in brain and blood and plasma butyrylcholinesterase activity (BChE) were monitored at 2 and 72 h after intoxication. Enzyme activity was determined using Ellman's colorimetric method.</p><p><strong>Results: </strong>The applied therapies with OBID, ATR and NAC in various configurations significantly reactivated PM-inhibited AChE in the brain and erythrocytes and the BChE in the plasma. The benefits of NAC administration in combination with ATR and/or OBID therapy have also been reported to restore AChE activity in the brain. NAC may reduce the dose of ATR in the treatment of PM poisoning.</p><p><strong>Conclusions: </strong>Adjunctive treatment offered by NAC can reduce or prevent the deleterious effects against PM-induced toxicity. Therefore, NAC remains a strong candidate for adjunct treatment for OP-poisoning, including PM, although additional preclinical and clinical studies are needed.</p>\",\"PeriodicalId\":50970,\"journal\":{\"name\":\"Annals of Agricultural and Environmental Medicine\",\"volume\":\"32 1\",\"pages\":\"116-121\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-03-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Agricultural and Environmental Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.26444/aaem/199716\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Agricultural and Environmental Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.26444/aaem/199716","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/14 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
The effect of the antioxidant N-acetylcysteine on cholinesterase activity in the brain and blood during Pirimiphos methyl poisoning in the course of treatment with atropine alone, and with atropine and obidoxime.
Introduction and objective: The antioxidant N-acetylcysteine (NAC) may help in the treatment of organophosphates poisoning, including Pirymiphos methyl (PM). However, there is no information on the effect of NAC on target cholinesterases during the core treatment with atropine and obidoxime after acute and chronic exposure to PM. The impact was investigated of NAC on the functional status of target cholinesterases in the brain and blood during treatment with atropine (ATR) and/or obidoxime (OBID) in PM-induced toxicity.
Material and methods: All experiments were performed on Male Swiss mice. The animals were intoxicated with PM and treated with OBID and/or ATR with or without and NAC, in various combinations (with 2-3 drugs) used simultaneously after intoxication. Total acetylcholinesterase activity (AChE) in brain and blood and plasma butyrylcholinesterase activity (BChE) were monitored at 2 and 72 h after intoxication. Enzyme activity was determined using Ellman's colorimetric method.
Results: The applied therapies with OBID, ATR and NAC in various configurations significantly reactivated PM-inhibited AChE in the brain and erythrocytes and the BChE in the plasma. The benefits of NAC administration in combination with ATR and/or OBID therapy have also been reported to restore AChE activity in the brain. NAC may reduce the dose of ATR in the treatment of PM poisoning.
Conclusions: Adjunctive treatment offered by NAC can reduce or prevent the deleterious effects against PM-induced toxicity. Therefore, NAC remains a strong candidate for adjunct treatment for OP-poisoning, including PM, although additional preclinical and clinical studies are needed.
期刊介绍:
All papers within the scope indicated by the following sections of the journal may be submitted:
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases).
Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and water.
Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dust.
Prevention of occupational diseases in agriculture, forestry, food industry and wood industry.
Work-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and prevention.
State of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.