{"title":"皮肤印迹法用于早期脱水检测的快速皮肤牛磺酸测定装置的研制与评价","authors":"Haruka Tsuchiya, Mari Abe, Sanai Tomida, Shiho Higashimura, Daijiro Haba, Takeo Minematsu, Hiromi Sanada, Gojiro Nakagami","doi":"10.1111/jjns.70007","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>Skin blotting is a noninvasive method to collect molecules like skin taurine, a dehydration indicator, by applying a membrane to the skin. However, quantifying skin taurine takes an hour due to the long process of staining and measurement. This study aimed to determine optimal staining conditions and assess the reliability and validity of spraying and light transmittance as new methods for skin blotting. Image analysis was used to evaluate the impact of these methods.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This study consisted of two parts. Study 1 focused on determining taurine staining conditions on the anion exchange membrane. Ethanol concentration for dissolving ninhydrin and spray time were optimized using a standard sample. Light transmittance and image analysis were used to quantify taurine. The relationship between taurine concentration and light transmittance or brightness (from image analysis) was evaluated. Study 2 confirmed the reliability and validity of the new methods using human samples. Reliability was assessed using the intraclass correlation coefficient (ICC), and validity was determined by correlation coefficients between taurine levels quantified by the conventional method with those quantified by light transmittance or image analysis.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The optimal spray time and ethanol concentration were 0.5 s and 90%, respectively, reducing measurement time to 7 min (light transmittance) and 10 min (image analysis). The ICC was 0.48 for light transmittance and 0.81 for image analysis. The correlation coefficients were <i>r</i> = 0.530 for light transmittance and <i>r</i> = 0.609 for image analysis.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Image analysis, which measures a wider area, showed better reliability and validity in quantifying skin taurine.</p>\n </section>\n </div>","PeriodicalId":50265,"journal":{"name":"Japan Journal of Nursing Science","volume":"22 2","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jjns.70007","citationCount":"0","resultStr":"{\"title\":\"Development and evaluation of a rapid skin taurine measurement device using skin blotting for the early detection of dehydration\",\"authors\":\"Haruka Tsuchiya, Mari Abe, Sanai Tomida, Shiho Higashimura, Daijiro Haba, Takeo Minematsu, Hiromi Sanada, Gojiro Nakagami\",\"doi\":\"10.1111/jjns.70007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>Skin blotting is a noninvasive method to collect molecules like skin taurine, a dehydration indicator, by applying a membrane to the skin. However, quantifying skin taurine takes an hour due to the long process of staining and measurement. This study aimed to determine optimal staining conditions and assess the reliability and validity of spraying and light transmittance as new methods for skin blotting. Image analysis was used to evaluate the impact of these methods.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>This study consisted of two parts. Study 1 focused on determining taurine staining conditions on the anion exchange membrane. Ethanol concentration for dissolving ninhydrin and spray time were optimized using a standard sample. Light transmittance and image analysis were used to quantify taurine. The relationship between taurine concentration and light transmittance or brightness (from image analysis) was evaluated. Study 2 confirmed the reliability and validity of the new methods using human samples. Reliability was assessed using the intraclass correlation coefficient (ICC), and validity was determined by correlation coefficients between taurine levels quantified by the conventional method with those quantified by light transmittance or image analysis.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The optimal spray time and ethanol concentration were 0.5 s and 90%, respectively, reducing measurement time to 7 min (light transmittance) and 10 min (image analysis). The ICC was 0.48 for light transmittance and 0.81 for image analysis. The correlation coefficients were <i>r</i> = 0.530 for light transmittance and <i>r</i> = 0.609 for image analysis.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Image analysis, which measures a wider area, showed better reliability and validity in quantifying skin taurine.</p>\\n </section>\\n </div>\",\"PeriodicalId\":50265,\"journal\":{\"name\":\"Japan Journal of Nursing Science\",\"volume\":\"22 2\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jjns.70007\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japan Journal of Nursing Science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jjns.70007\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NURSING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japan Journal of Nursing Science","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jjns.70007","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NURSING","Score":null,"Total":0}
Development and evaluation of a rapid skin taurine measurement device using skin blotting for the early detection of dehydration
Aim
Skin blotting is a noninvasive method to collect molecules like skin taurine, a dehydration indicator, by applying a membrane to the skin. However, quantifying skin taurine takes an hour due to the long process of staining and measurement. This study aimed to determine optimal staining conditions and assess the reliability and validity of spraying and light transmittance as new methods for skin blotting. Image analysis was used to evaluate the impact of these methods.
Methods
This study consisted of two parts. Study 1 focused on determining taurine staining conditions on the anion exchange membrane. Ethanol concentration for dissolving ninhydrin and spray time were optimized using a standard sample. Light transmittance and image analysis were used to quantify taurine. The relationship between taurine concentration and light transmittance or brightness (from image analysis) was evaluated. Study 2 confirmed the reliability and validity of the new methods using human samples. Reliability was assessed using the intraclass correlation coefficient (ICC), and validity was determined by correlation coefficients between taurine levels quantified by the conventional method with those quantified by light transmittance or image analysis.
Results
The optimal spray time and ethanol concentration were 0.5 s and 90%, respectively, reducing measurement time to 7 min (light transmittance) and 10 min (image analysis). The ICC was 0.48 for light transmittance and 0.81 for image analysis. The correlation coefficients were r = 0.530 for light transmittance and r = 0.609 for image analysis.
Conclusions
Image analysis, which measures a wider area, showed better reliability and validity in quantifying skin taurine.
期刊介绍：
The Japan Journal of Nursing Science is the official English language journal of the Japan Academy of Nursing Science. The purpose of the Journal is to provide a mechanism to share knowledge related to improving health care and promoting the development of nursing. The Journal seeks original manuscripts reporting scholarly work on the art and science of nursing. Original articles may be empirical and qualitative studies, review articles, methodological articles, brief reports, case studies and letters to the Editor. Please see Instructions for Authors for detailed authorship qualification requirement.
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