Genome-wide identification and expression profiling of the hemoglobin gene family under hypoxia stress in Sillago sihama

Hemoglobin (Hb) plays a pivotal role in oxygen transport and is essential for the adaptive response to hypoxic stress. Studying the molecular evolution characteristics and expression pattern of S. sihama Hb gene family can provide theoretical basis for further revealing the roles of the Hb genes against hypoxia stress. This study aimed to identify the molecular evolution characteristics and expression patterns of Hb genes in S. sihama. Utilizing bioinformatics methods, we identified ten members of the Hb gene family within the whole genome of S. sihama, located on chromosomes LG16 and LG21 in a tandem repeat pattern. Phylogenetic analysis classified the Hb gene family into two subfamilies (alpha and beta), showing close relationships with the Hb gene family members of the large yellow croaker (Larimichthys crocea). Both phylogenetic and collinearity analysis indicated the conservation of Hb genes throughout evolution. The nonsynonymous substitution rate / synonymous substitution rate (Ka/Ks) results showed that its evolution was purifying selection. RNA-seq and qRT-PCR analyses revealed that five Hb genes were highlighted high expression levels in the heart and liver of S. sihama, with sshba1.4, sshbad, and sshba1 showing significant up-regulation in response to hypoxic stress. In summary, this study highlights the vital role of the Hb gene family in the hypoxic stress response of S. sihama, suggesting their potential as crucial candidate genes for regulating environmental stress in this species.