Diagnostic value and regulatory role of long non-coding RNA MIR210HG in hypertension.

Background: Hypertension is the most prevalent chronic disease globally and the treatment and control ratio of hypertension patients are still low. The function of lncRNA MIR210HG in hypertension has not yet been announced.

Methods: Eighty-three hypertension patients and 79 healthy individuals were enrolled. The Human Umbilical Vein Endothelial Cells (HUVECs) treated with Ang II were employed to imitate hypertension. The relative expression of MIR210HG and miR-125b-5p were evaluated by qRT-PCR while the ROC curve was applied to assess the diagnostic performance of MIR210HG. The CCK-8 assay was performed to detect the proliferation ability. The transwell assay and flow cytometry were used to analyze the migratory or apoptosis, respectively. The dual luciferase reporter system was used to confirm the targeted relationship between MIR210HG and miR-125b-5p.

Results: Up-regulated MIR210HG was found in hypertension patients (P<0.001) compared to healthy individuals and the upregulation was positively associated with the severity of hypertension (P<0.01). Up-regulated MIR210HG exhibited a promising diagnosability for hypertension patients. The area under the ROC curve was 0.8765 with high sensitivity (81.93%) and specificity (83.28%). In hypertension cell model, increased MIR210HG was observed along with declined cell proliferation, migration and enhanced apoptosis, which were reversed by MIR210HG knockdown. MIR210HG knockdown also inhibited inflammation levels including TNF-α, IL-1β and IL-6. The miR-125b-5p was a potential downstream target of MIR210HG and they were negatively correlated in expression.

Conclusions: Up-regulated MIR210HG was a promising diagnostic biomarker in identifying hypertension patients. MiR-125b-5p was a potential downstream target of MIR210HG in HUVECs.