一种基于数字图像的方法来筛选调节黑色素化的分子。

IF 1.7 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Waka Shimosako, Susumu Tanimura, Taiki Baba, Megumi Kuroiwa, Hiroyuki Murota, Kohsuke Takeda
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引用次数: 0

摘要

寻常白癜风是一种后天疾病,被认为是由基底表皮层黑色素细胞抑制黑色素合成引起的。为了开发治疗寻常白癜风的药物,鉴定促进黑色素化的化合物是至关重要的。在这项研究中,我们建立了一种基于数字图像的方法来量化黑化,而不需要生化程序。B16F10细胞接种于96孔白底微孔板。用α-促黑素激素或不加α-促黑素激素处理后,将细胞固定,采集微孔板的数字图像,测量图像上各孔的总信号强度。在从相应的空白孔中减去信号后,定义每个孔中细胞的黑色素化程度。这种方法被发现比测量细胞裂解物在UV-A波长的吸光度的传统技术更敏感地量化黑化。我们得到的统计参数表明,该方法适用于高通量筛选试验;因此,这种方法似乎对筛选和鉴定抑制或促进黑色素化的分子是有用的,后者可能被开发为治疗寻常白癜风的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a Digital Image-Based Method to Screen Molecules That Regulate Melanization.

Vitiligo vulgaris is an acquired disorder that is thought to arise from the suppression of melanin synthesis by melanocytes in the basal epidermal layer. To develop therapeutic agents for vitiligo vulgaris, it is critical to identify compounds that promote melanization. In this study, we established a digital image-based method to quantify melanization that does not require biochemical procedures. B16F10 cells were seeded in a white-bottom 96-well microplate. After treatment with or without α-melanocyte-stimulating hormone, followed by fixation of the cells, digital images of the microplates were captured, and the total signal intensity of each well on the image was measured. The extent of melanization in the cells in each well was defined after the subtraction of the signal from the corresponding blank well. This method was found to quantify melanization more sensitively than the conventional technique that measures the absorbance of cell lysates at UV-A wavelengths. We obtained statistical parameters showing that this method was applicable to a high-throughput screening assay; thus, this method appears to be useful for screening and identifying molecules that suppress or promote melanization, the latter of which may be developed as therapeutic agents for vitiligo vulgaris.

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来源期刊
CiteScore
3.50
自引率
5.00%
发文量
247
审稿时长
2 months
期刊介绍: Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.
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