lncRNA NR_146969促进肺腺癌的进展

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Experimental cell research Pub Date : 2025-03-25 DOI:10.1016/j.yexcr.2025.114535

Ying Chen , Juan Wang , Linguo Gu , Hongzuo Chen , Zhengling Gai , Rui Hu , Bei Qing , Yunchang Yuan , Zhenkun Xia

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引用次数: 0

摘要

越来越多的研究表明，长链非编码rna （lncRNAs）的失调与癌症的发生和发展密切相关。在本研究中，我们使用lncRNA阵列技术鉴定肺腺癌患者和正常肺组织中差异表达的lncRNA。本研究进一步探讨了候选lncrna在肺腺癌（LUAD）中的临床意义和功能。结果显示lncRNA NR_146969在LUAD标本中表达上调，且与LUAD患者淋巴结转移及临床分期相关。方法采用CCK-8、集落形成、transwell实验和异种移植肿瘤模型等方法观察lncRNA NR_146969的生物学功能。通过FISH、双荧光素酶报告基因试验和回收率试验探索lncRNA NR_146969的潜在作用机制。结果总的来说，lncRNA NR_146969在LUAD中起致癌作用。机械上，lncRNA NR_146969通过miR-26a-1-3p靶向SLC6A14，导致AKT/mTOR通路磷酸化，从而促进LUAD的生长和转移。结论靶向lncRNA NR_146969可能为LUAD提供新的治疗策略。

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lncRNA NR_146969 promotes the progression of lung adenocarcinoma

Background

Emerging research suggests that dysregulation of long non-coding RNAs (lncRNAs) is closely linked to the onset and progression of cancer. In this study, we used lncRNA array technology to identify differentially expressed lncRNAs in lung adenocarcinoma patients and normal lung tissues. The study further explored the clinical significance and function of candidate lncRNAs in lung adenocarcinoma (LUAD). The results showed that lncRNA NR_146969 was upregulated in LAUD specimens and was associated with lymph node metastasis and clinical staging in LUAD patients.

Methods

The biological functions of lncRNA NR_146969 were observed using CCK-8, colony formation, transwell assay and xenograft tumor model. Explore the potential mechanism of action of lncRNA NR_146969 by FISH, dual luciferase reporter assay and recovery assay.

Results

Overall, lncRNA NR_146969 plays an oncogenic role in LUAD. Mechanically, lncRNA NR_146969 targets SLC6A14 via miR-26a-1-3p, leading to phosphorylation of the AKT/mTOR pathway, which promotes LUAD growth and metastasis.

Conclusion

Therefore, targeting lncRNA NR_146969 may provide a new therapeutic strategy for LUAD.

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来源期刊

Experimental cell research 医学-细胞生物学

CiteScore

7.20

自引率

0.00%

发文量

295

审稿时长

30 days

期刊介绍： Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.