结合两种重组酶OleT-ELP和HMFO-ELP同时生产α-烯烃和2,5-呋喃二羧酸

IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yaqi Fu , Siyu Mao , Tianyue Liao , Wei Feng
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引用次数: 0

摘要

OleT酶可以利用H2O2作为共底物,这种生物催化是一种H2O2驱动的酶催化。在这项工作中,OleT通过与弹性蛋白样多肽(ELP)融合而重组。与天然OleT相比,重组酶OleT- elp具有更高的稳定性和抗H2O2干扰能力。OleT-ELP对脂肪酸脱羧法制备α-烯烃具有较高的催化效率。重组5-羟甲基糠醛氧化酶(HMFO-ELP)催化5-羟甲基糠醛(HMF)氧化生成2,5-呋喃二羧酸(FDCA),生成H2O2作为副产物。将OleT-ELP与HMFO-ELP结合,可以实现脂肪酸和HMF的同时转化。HMFO-ELP生成的原位H2O2被转移到OleT-ELP上,提高了α-烯烃和FDCA的催化效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simultaneous production of linear α-olefins and 2,5-furandicarboxylic acid by combining two recombinant enzymes OleT-ELP and HMFO-ELP
The enzyme OleT can utilize H2O2 as the co-substrate, and this biocatalysis is an H2O2-driven enzymatic catalysis. In this work, OleT was recombinated by being fused to an elastin-like polypeptide (ELP). The recombinant enzyme OleT-ELP exhibits higher stability and resistance to H2O2 interference compared to native OleT. OleT-ELP showed improved catalytic efficiency in producing α-olefins via fatty acid decarboxylation. The recombinant 5-hydroxymethylfurfural oxidase (HMFO-ELP) catalyzes the oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA), generating H2O2 as a byproduct. Combining OleT-ELP with HMFO-ELP enabled simultaneous conversion of fatty acids and HMF. The in situ H2O2 generated by HMFO-ELP was transferred to OleT-ELP, enhancing catalytic efficiencies for both α-olefins and FDCA production.
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来源期刊
Enzyme and Microbial Technology
Enzyme and Microbial Technology 生物-生物工程与应用微生物
CiteScore
7.60
自引率
5.90%
发文量
142
审稿时长
38 days
期刊介绍: Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.
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