香蕉皮发酵液作为酸性矿山废水生物硫酸盐还原的可行替代碳源

IF 2.8 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2025-02-03 DOI:10.1002/jctb.7822

Yu Gao, Jiaxing Li, Qintong Li, Rui Sun, Wenxin Hua, Chengze Wang, Jianliang Xue, Ping Chen

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引用次数: 0

摘要

酸性矿井水（AMD）由于其强酸性、高浓度的硫酸盐和重金属，是矿区主要的环境威胁。利用硫酸盐还原菌（SRB）减少SO42−和去除重金属是一种有前途的经济的AMD处理方法。然而，AMD缺乏足够的电子给体来还原硫酸盐。香蕉皮发酵液含有多种小分子有机酸，是SRB的有效替代碳源，可促进农业废弃物在环境修复中的利用。本研究旨在研究在生物反应器中添加香蕉皮发酵液对污染物去除性能的影响，并分析其对微生物群落结构的影响。结果以香蕉皮发酵液为电子供体的生物反应器对硫酸盐（55.7%）、Cu2+(90.74%)、Mn2+(70.77%)、Fe2+(81.28%)和Cd2+(100.00%)等污染物均有较好的去除率，化学需氧量（COD）平均降低74.20%。硫酸盐还原导致pH从5.5增加到7.9。微生物群落结构分析表明，参与硫酸盐还原的主要属为脱硫属和脱硫弧菌属，而参与重金属固定化的主要属为Delftia属。此外，负责发酵产生小分子酸的关键属是Leptolinea和Sedimentibacter。它们与SRB共生，对金属和硫酸盐的去除效率起着至关重要的作用。结论反应器连续运行30 d后，总体硫酸盐和重金属去除效果满意。反应器内微生物丰度和群落多样性显著增加。因此，香蕉皮发酵液是一种很有前途的酸性矿山废水处理有机碳源。©2025化学工业学会（SCI）。

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Banana peel fermentation broth as a viable alternative carbon source for biological sulfate reduction in acid mine drainage

BACKGROUND

Acid mine drainage (AMD) is a major environmental threat in mining areas due to its strong acidity, high concentrations of sulfate, and heavy metals. Utilizing sulfate-reducing bacteria (SRB) to reduce SO₄²⁻ and remove heavy metals is a promising and economical AMD treatment. However, AMD lacks sufficient electron donors for sulfate reduction. Banana peel fermentation broth, containing various small-molecule organic acids, is an effective alternative carbon source for SRB, promoting the use of agricultural waste in environmental remediation. This study aims to investigate the impact of adding banana peel fermentation broth to the bioreactor on pollutant removal performance and analyze its effect on the microbial community structure.

RESULTS

The bioreactor utilising banana peel fermentation broth as an electron donor, demonstrated the effective removal of various pollutants, including sulfate (55.7%), Cu²⁺ (90.74%), Mn²⁺ (70.77%), Fe²⁺ (81.28%) and Cd²⁺ (100.00%), with an average reduction of 74.20% in chemical oxygen demand (COD). Sulfate reduction resulted in an increase in pH from 5.5 to 7.9. Microbial community structure analysis revealed that the primary genera involved in sulfate reduction were Desulfurispora and Desulfovibrio, while Delftia contributes to the immobilization of heavy metals. Additionally, the key genera responsible for fermentation to produce small molecule acids were Leptolinea and Sedimentibacter. In symbiosis with SRB, they play a crucial role in the removal efficiency of metals and sulfate.

CONCLUSION

The overall sulfate and heavy metals removal efficiency was found to be satisfactory after 30 days of continuous reactor operation. There was a notable increase in microbial abundance and community diversity within the reactor. Therefore, banana peel fermentation broth is a promising alternative organic carbon source in acid mine drainage(AMD) treatment. © 2025 Society of Chemical Industry (SCI).

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.