海狸坝类似物对水生原生动物病原体十二指肠贾第鞭毛虫和细小隐孢子虫的影响。

IF 3.9 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ariel I Loredo, Andrea Packham, Nick Graham, Skylar Johnson, Stephanie Elliott, Brian H Bird, Carrie Monohan, Sarah Yarnell, Woutrina A Smith
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引用次数: 0

摘要

在淡水生态系统中安装海狸坝模拟物(BDAs)是一种基于过程的修复技术,它模仿天然海狸坝,以增加河道的复杂性、洪泛平原的连通性和水文停留时间。近年来,资源管理者已将生物多样性开发用于改善河岸生境,对生态系统服务产生有益影响,包括改善水质。虽然BDA已被证明可以减少地表水中的悬浮沉积物和营养物质,但还需要研究评估BDA对十二指肠贾第鞭毛虫(与蓝氏贾第鞭毛虫、肠道贾第鞭毛虫)和细小隐孢子虫等水媒粪便病原体的影响。在加利福尼亚的一个河岸场地,本研究使用了bda上游灭活原生动物(oo)囊的原位释放试验,以比较bda上方和下方检测到的浓度和负荷。在bda位点,十二指肠梭菌的负荷量回收率中位数为78%,小梭菌的负荷量回收率中位数为80%。混合效应线性回归分析显示,与不含bda的类似部位相比,含有bda的下游地表水中十二指肠螺杆菌调节的囊肿浓度显著降低(81%)。虽然注意到小孢子虫卵囊的减少,但没有统计学意义。BDA结构对下游地表水水质的改善可能是由于BDA结构促进了潜流交换,并起到了去除溪流中病原原生动物的被动过滤器的作用。如果bda被用来帮助河岸草甸生态系统的恢复,以减缓水流和提高地下水位,它们也可能有助于促进下游社区获得更清洁和更安全的水。重要性:海狸坝类似物(BDAs)是一种具有成本效益,低环境影响的溪流和河岸草甸恢复技术,提供各种有益的生态系统服务;然而,它们对水传播的粪便原生动物病原体的影响尚未得到评估。本研究采用原位原生动物释放试验来量化BDAs对加利福尼亚溪流中十二指肠贾第虫和小隐孢子虫负荷的影响。结果表明,在bda水平下,十二指肠菌浓度显著降低81%。十二指肠梭菌和小梭菌在低于BDA的负荷回收率中位数分别为78%和80%。这一发现表明,BDAs可能会促进水传播病原体的被动过滤,从而改善下游水质,并增加BDAs在溪流和草甸恢复工作中的有益效果。对资源管理者和社区的潜在好处是巨大的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of beaver dam analogs (BDAs) on waterborne protozoal pathogens Giardia duodenalis and Cryptosporidium parvum.

Installing beaver dam analogs (BDAs) in freshwater ecosystems is a process-based restoration technique mimicking natural beaver dams to increase stream channel complexity, floodplain connectivity, and hydrological residence time. BDAs have been applied in recent years by resource managers to improve riparian habitats with beneficial effects on ecosystem services, including improved water quality. While BDAs have been shown to reduce suspended sediments and nutrients in surface waters, research is needed to evaluate BDA effects on waterborne fecal pathogens such as Giardia duodenalis (syn. G. lamblia, G. intestinalis) and Cryptosporidium parvum. At a California riparian field site, this study used an in situ release trial of inactivated protozoal (oo)cysts upstream of BDAs to compare the concentrations and loads detected above and below the BDAs. The median percent decrease in load-based recovery rates was 78% for G. duodenalis and 80% for C. parvum at sites with BDAs. Mixed-effect linear regression analyses showed a significant reduction (by 81%) of the G. duodenalis adjusted cyst concentrations in downstream surface waters at sites with BDAs compared to similar sites without BDAs. While a reduction of C. parvum oocysts was noted, it was not statistically significant. The improved surface water quality downstream of BDAs could be because the BDA structures promote hyporheic exchange and act as a passive filter to remove pathogenic protozoa from streams. If BDAs are used to aid the restoration of riparian meadow ecosystems to slow water and raise the water table, they may also help to promote cleaner and safer waters for communities downstream.

Importance: Beaver dam analogs (BDAs) are a cost-effective, low-environmental impact technique for stream and riparian meadow restoration that provide a variety of beneficial ecosystem services; however, their impact on waterborne fecal protozoal pathogens has not been evaluated. This study used an in situ protozoal release trial to quantify the effect of BDAs on the load of Giardia duodenalis and Cryptosporidium parvum in streams in California. Results showed that G. duodenalis concentrations below BDAs were significantly reduced by 81%. The median percent decrease in load-based recovery rates below a BDA for G. duodenalis and C. parvum was 78% and 80%, respectively. This finding indicates that BDAs may promote passive filtration of waterborne pathogens, thereby improving water quality in downstream reaches and adding to the beneficial outcomes associated with using BDAs in stream and meadow restoration efforts. The potential benefit to resource managers and communities is immense.

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来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
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