Wangbo Chen, Sun Xu, Shuzhen Zou, Zijian Liu, Yichi Liu, Haozhe Xu, Jiayue Wang, Junjie Ma, Rong Chen, Zhaojiang Zuo
{"title":"不同光照条件下铜绿微囊藻生产生物燃料的碳水化合物和脂质产量","authors":"Wangbo Chen, Sun Xu, Shuzhen Zou, Zijian Liu, Yichi Liu, Haozhe Xu, Jiayue Wang, Junjie Ma, Rong Chen, Zhaojiang Zuo","doi":"10.1186/s13068-025-02615-8","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Biofuels produced from algae have enormous advantages in replacing fossil fuels, and <i>Microcystis aeruginosa</i> has a great potential for biofuel production, due to growing fast to form large amounts of biomass. Light is essential for algal growth, and the optimum light quality can promote the biomass and lipid accumulation for increasing feedstock for biofuel production.</p><h3>Results</h3><p>We investigated the biomass accumulation, photosynthetic ability, carbohydrate, and lipid yield as well as related gene expression in <i>M. aeruginosa</i> under red, blue, purple, and white light to promote biofuel production using this alga under the optimal light quality. Compared with white light, purple light promoted the cell growth during the 5 days, while blue light showed inhibitory effect. Red light had no effect on the cell growth, but improved the biomass content to the highest level. Red light improved the photosynthetic ability by raising chlorophyll level, and up-regulating expression of the genes in chlorophyll biosynthesis, photosynthetic electron transfer, and CO<sub>2</sub> fixation. Among these light qualities, red light showed the maximum effect on soluble, insoluble, and total carbohydrate accumulation by up-regulating the genes in polysaccharide and starch formation, and down-regulating the genes in glycolysis and tricarboxylic acid cycle. Red light also exhibited the maximum effect on lipid accumulation, which might be caused by up-regulating five genes in fatty acid biosynthesis.</p><h3>Conclusion</h3><p>Red light can promote <i>M. aeruginosa</i> accumulating carbohydrates and lipids by regulating related gene expression, which should be the optimal light quality for improving feedstock yield for biofuel production.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02615-8","citationCount":"0","resultStr":"{\"title\":\"Carbohydrate and lipid yield in Microcystis aeruginosa for biofuel production under different light qualities\",\"authors\":\"Wangbo Chen, Sun Xu, Shuzhen Zou, Zijian Liu, Yichi Liu, Haozhe Xu, Jiayue Wang, Junjie Ma, Rong Chen, Zhaojiang Zuo\",\"doi\":\"10.1186/s13068-025-02615-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Biofuels produced from algae have enormous advantages in replacing fossil fuels, and <i>Microcystis aeruginosa</i> has a great potential for biofuel production, due to growing fast to form large amounts of biomass. 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Carbohydrate and lipid yield in Microcystis aeruginosa for biofuel production under different light qualities
Background
Biofuels produced from algae have enormous advantages in replacing fossil fuels, and Microcystis aeruginosa has a great potential for biofuel production, due to growing fast to form large amounts of biomass. Light is essential for algal growth, and the optimum light quality can promote the biomass and lipid accumulation for increasing feedstock for biofuel production.
Results
We investigated the biomass accumulation, photosynthetic ability, carbohydrate, and lipid yield as well as related gene expression in M. aeruginosa under red, blue, purple, and white light to promote biofuel production using this alga under the optimal light quality. Compared with white light, purple light promoted the cell growth during the 5 days, while blue light showed inhibitory effect. Red light had no effect on the cell growth, but improved the biomass content to the highest level. Red light improved the photosynthetic ability by raising chlorophyll level, and up-regulating expression of the genes in chlorophyll biosynthesis, photosynthetic electron transfer, and CO2 fixation. Among these light qualities, red light showed the maximum effect on soluble, insoluble, and total carbohydrate accumulation by up-regulating the genes in polysaccharide and starch formation, and down-regulating the genes in glycolysis and tricarboxylic acid cycle. Red light also exhibited the maximum effect on lipid accumulation, which might be caused by up-regulating five genes in fatty acid biosynthesis.
Conclusion
Red light can promote M. aeruginosa accumulating carbohydrates and lipids by regulating related gene expression, which should be the optimal light quality for improving feedstock yield for biofuel production.
期刊介绍：
Biotechnology for Biofuels is an open access peer-reviewed journal featuring high-quality studies describing technological and operational advances in the production of biofuels, chemicals and other bioproducts. The journal emphasizes understanding and advancing the application of biotechnology and synergistic operations to improve plants and biological conversion systems for the biological production of these products from biomass, intermediates derived from biomass, or CO2, as well as upstream or downstream operations that are integral to biological conversion of biomass.
Biotechnology for Biofuels focuses on the following areas:
• Development of terrestrial plant feedstocks
• Development of algal feedstocks
• Biomass pretreatment, fractionation and extraction for biological conversion
• Enzyme engineering, production and analysis
• Bacterial genetics, physiology and metabolic engineering
• Fungal/yeast genetics, physiology and metabolic engineering
• Fermentation, biocatalytic conversion and reaction dynamics
• Biological production of chemicals and bioproducts from biomass
• Anaerobic digestion, biohydrogen and bioelectricity
• Bioprocess integration, techno-economic analysis, modelling and policy
• Life cycle assessment and environmental impact analysis
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