基于串联质谱（MS2）的糖蛋白组学研究现状及其补充

IF 3 Q4 Biochemistry, Genetics and Molecular Biology

BBA Advances Pub Date : 2025-01-01 DOI:10.1016/j.bbadva.2025.100158

Hiroyuki Kaji

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引用次数: 0

摘要

随着支持蛋白质组学的液相色谱/质谱的发展以及相关分析方法和数据分析软件的开发，可以在单次分析中识别和量化的蛋白质数量正在接近转录组学的水平。然而，在蛋白质糖基化分析中仍有许多问题有待解决。这篇综述讨论了基于ms2的糖蛋白鉴定的技术问题，以及日本人类糖蛋白图谱（HGA）项目对其的补充。

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Current issues of tandem mass spectrum (MS2)-based glycoproteomics and efforts to complement them

With the development of liquid chromatography/mass spectrometers that support proteomics and the associated development of analytical methods and data analysis software, the number of proteins that can be identified and quantified in a single analysis is approaching the level of transcriptomics. However, many problems remain to be solved in protein glycosylation analysis. This mini-review discusses the technical issues of MS2-based glycoprotein identification and efforts to complement them by the Human Glycome Atlas (HGA) Project in Japan.

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来源期刊

BBA Advances Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

10 weeks