利用自标记蛋白开发共价化学标记。

IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nicola Porzberg, Klara Gries, Kai Johnsson
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引用次数: 0

摘要

活细胞中蛋白质的可视化和操作对于研究复杂的生物过程至关重要。自标记蛋白通过合成探针的特异性和共价附着来实现这一点,为活细胞和体内蛋白质的化学标记提供了前所未有的灵活性。通过将合成染料优异的光物理特性与遗传靶向性相结合,这些标签为活细胞和高分辨率荧光成像提供了模块化和创新的工具箱。本文综述了自标记蛋白关键技术HaloTag7、SNAP-tag、CLIP-tag以及共价甲氧嘧啶(TMP)-tag的发展及其应用。我们讨论了最近在蛋白质工程和底物设计方面的创新,这些创新引入了新的功能,以实现多路成像,超分辨率显微镜以及新型生物传感器和记录仪的设计。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploiting Covalent Chemical Labeling with Self-Labeling Proteins.

The visualization and manipulation of proteins in live cells are critical for studying complex biological processes. Self-labeling proteins do so by enabling the specific and covalent attachment of synthetic probes, offering unprecedented flexibility in the chemical labeling of proteins in live cells and in vivo. By combining the excellent photophysical properties of synthetic dyes with genetic targetability, these tags provide a modular and innovative toolbox for live-cell and high-resolution fluorescence imaging. In this review, we explore the development and diverse applications of the key self-labeling protein technologies, HaloTag7, SNAP-tag, and CLIP-tag, as well as the covalent trimethoprim (TMP)-tag. We discuss recent innovations in both protein engineering and substrate design that have introduced new functionalities to enable multiplexed imaging, super-resolution microscopy, and the design of novel biosensors and recorders.

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来源期刊
Annual review of biochemistry
Annual review of biochemistry 生物-生化与分子生物学
CiteScore
33.90
自引率
0.00%
发文量
31
期刊介绍: The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.
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