TCL-E5和TCL-E5脉冲dc的免疫治疗作用:两种新的HPV治疗性候选疫苗

IF 2.7 4区 医学 Q3 IMMUNOLOGY
Immunotherapy Pub Date : 2025-02-01 Epub Date: 2025-03-18 DOI:10.1080/1750743X.2025.2478814
Fahimeh Ezzatizadeh, Azam Bolhassani, Fattah Sotoodehnejad Nematalahi, Abolfazl Fateh
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引用次数: 0

摘要

目的:研究HPV16 E5肿瘤蛋白修饰的肿瘤细胞裂解液(TCL-E5)和TCL-E5脉冲树突状细胞(DCs)增强小鼠抗肿瘤免疫的潜力。材料与方法:用携带E5蛋白的慢病毒颗粒转染TC1肿瘤细胞,生成TCL-E5。此外,用TCL-E5脉冲DCs制备细胞上清液。用小鼠模型研究了它们的免疫应答和抗肿瘤作用。结果:tcl - e5脉冲dc方案可引导免疫应答Th1和CTL,导致肿瘤体积缩小,无肿瘤小鼠比例高。结论:与tcl -E5脉冲DCs方案相比,tcl脉冲DCs方案不能诱导显著的抗肿瘤作用,提示E5在疫苗研制中的主要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunotherapeutic effects of TCL-E5 and TCL-E5-pulsed DCs: two novel HPV therapeutic vaccine candidates.

Aim: This study investigated the potential of HPV16 E5 oncoprotein-modified tumor cell lysate (TCL-E5) and dendritic cells (DCs) pulsed with TCL-E5 (TCL-E5-pulsed DCs) to enhance antitumor immunity in a murine model.

Materials and methods: For generation of TCL-E5, TC1 tumor cells were transduced with lentiviral particles harboring E5 protein. Moreover, the cell supernatants were prepared from DCs pulsed with TCL-E5. Their immunological responses and antitumor effects were investigated in a mouse model.

Results: The TCL-E5-pulsed DCs regimen could direct immunity toward Th1 and CTL responses, leading to tumor volume reduction and high percentage of tumor-free mice.

Conclusion: The TCL-pulsed DCs regimen could not induce significant antitumor effects compared to TCL-E5-pulsed-DCs regimen indicating main role of E5 in vaccine development.

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来源期刊
Immunotherapy
Immunotherapy 医学-免疫学
CiteScore
5.00
自引率
3.60%
发文量
113
审稿时长
6-12 weeks
期刊介绍: Many aspects of the immune system and mechanisms of immunomodulatory therapies remain to be elucidated in order to exploit fully the emerging opportunities. Those involved in the research and clinical applications of immunotherapy are challenged by the huge and intricate volumes of knowledge arising from this fast-evolving field. The journal Immunotherapy offers the scientific community an interdisciplinary forum, providing them with information on the most recent advances of various aspects of immunotherapies, in a concise format to aid navigation of this complex field. Immunotherapy delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this vitally important area of research. Unsolicited article proposals are welcomed and authors are required to comply fully with the journal''s Disclosure & Conflict of Interest Policy as well as major publishing guidelines, including ICMJE and GPP3.
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