青少年双歧杆菌通过焦亡衰减预防糖尿病引起的肝损伤。

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Experimental cell research Pub Date : 2025-03-15 DOI:10.1016/j.yexcr.2025.114518

Seyed Mohammadmahdi Meybodi , Mohammad Javad Rezazadeh Khabaz , Andia Vojdani , Zahra Nasiri , Seyed Amirhossein Mazhari , Farideh Akhlaghi Tabar , Sara Abdizadeh Javazm , Marzieh Owrang , Zahra Noori , Maryam Sadat Pishva , Parisa Badameh , Mohammad Hasan Maleki , Elham Nadimi

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引用次数: 0

摘要

背景：非酒精性脂肪性肝病（NAFLD）和非酒精性脂肪性肝炎（NASH）缺乏明确的治疗方法，通常在达到晚期之前仍无症状。脂质代谢和炎症管理使用益生菌如青少年双歧杆菌被建议缓解或抑制NAFLD的发展。因此，本研究旨在探讨青少年双歧杆菌治疗对减轻高脂肪饮食（HFD）和链脲佐菌素（STZ）诱导的NAFLD大鼠肝脏焦亡（一种炎症细胞死亡途径）的影响。方法：48只8周龄成年雄性大鼠随机分为4组。对饲喂高脂肪饮食（HFD）的大鼠以4×1010 CFU/天的剂量给予青春期双歧杆菌8周和16周。随后，采用定量聚合酶链式反应（qPCR）定量分析肝组织中Cas1、Cas3、Cas11、NLRP3、GSDMD、IL-1β、NF-κB等热亡相关基因的mRNA表达水平。还评估了不同组肝脏结构的组织病理学改变和体视学改变，以及脂质谱（FBG、TG、TC、HDL、LDL）和肝脏指数（ALT、AST、ALP、LDH）。结果：青少年双歧杆菌显著降低了NF-κB和热噬相关基因的表达水平。此外，这种益生菌有效地逆转了高脂肪饮食（HFD）对肝脏体积、库普弗细胞数量和肝细胞核的不利影响。此外，它还能改善饲喂HFD大鼠的血脂和肝脏指标。结论：本研究表明，补充B.青少年通过减轻焦亡来预防糖尿病引起的肝损伤。这些发现表明，青少年双歧杆菌可能是一种很有希望的治疗NAFLD及其相关并发症的方法，主要是通过调节与高脂肪饮食喂养的大鼠焦亡和炎症相关的关键基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Bifidobacterium adolescentis prevents diabetes-induced liver injury via pyroptosis attenuation

查看原文本刊更多论文

Bifidobacterium adolescentis prevents diabetes-induced liver injury via pyroptosis attenuation

Background

Non-alcoholic fatty liver disease (NAFLD), along with non-alcoholic steatohepatitis (NASH), lacks definitive therapy and typically remains asymptomatic until reaching advanced stages. Lipid metabolism and inflammation management using probiotics such as Bifidobacterium adolescentis is suggested to alleviate or suppress NAFLD development. Hence, this study aims to investigate the effects of Bifidobacterium adolescentis treatment on mitigating pyroptosis, an inflammatory cell death pathway, in the liver of rats with NAFLD induced by high-fat diet (HFD) and streptozotocin (STZ) administration.

Methods

Forty 8-week adult male Sprague Dawley rats were divided into four groups. Bifidobacterium adolescentis was administered for 8 and 16 weeks at 4 × 10¹⁰ CFU/day to rats fed a high-fat diet (HFD). Subsequently, the mRNA expression levels of pyroptotic-related genes including Cas1, Cas3, Cas11, NLRP3, GSDMD, IL-1β, and NF-κB were quantified in liver tissue using quantitative polymerase chain reaction (qPCR). Histopathological alterations and stereological changes in liver structure, as well as lipid profile (FBG, TG, TC, HDL, LDL), and liver indices (ALT, AST, ALP, LDH), were also evaluated across the different groups.

Results

Bifidobacterium adolescentis administration significantly reduced the expression levels of NF-κB and pyroptotic-related genes. Additionally, this probiotic effectively reversed the adverse effects of the high-fat diet (HFD) on liver volume, Kupffer cell numbers, and hepatocyte nuclei. Furthermore, it improved the lipid profile and liver indices of rats fed with the HFD.

Conclusion

This study demonstrates that B. adolescentis supplementation prevents diabetes-induced liver injury by attenuating pyroptosis. These findings suggest that Bifidobacterium adolescentis may be a promising therapeutic approach for managing NAFLD and its associated complications, primarily by modulating key genes associated with pyroptosis and inflammation in rats fed with a high-fat diet.

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来源期刊

Experimental cell research 医学-细胞生物学

CiteScore

7.20

自引率

0.00%

发文量

295

审稿时长

30 days

期刊介绍： Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.