异酸碱抑制NLRP3炎性体改善妊娠糖尿病。

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI:10.2478/aite-2025-0006
Li Pu, Li Chen, Kun Yu, Yueming Zhang, Caifeng Wang, Feizhou Jiang, Jia Shi, Jingjing Meng
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引用次数: 0

摘要

本研究旨在探讨异酸碱(IRN)对妊娠期糖尿病(GDM)的作用及其机制。将db/+小鼠随机分为GDM、GDM + IRN (20 mg/kg)和GDM + IRN (40 mg/kg) 4组。在妊娠第10天采用腹腔葡萄糖耐量试验(ipgts)和腹腔胰岛素耐量试验(IPITTs)评估血糖和胰岛素耐量。在妊娠第20天,采用酶联免疫吸附法(ELISA)、免疫印迹法和生化法检测胎盘炎症(肿瘤坏死因子[TNF]-α、白细胞介素[IL]-6、IL-1β)、氧化应激标志物(丙二醛[MDA]、SOD、谷胱甘肽过氧化物酶[GPx]、谷胱甘肽[GSH])和核因子-κB/ nod样受体蛋白3 [NLRP3]炎性体活性。IRN显著改善GDM小鼠的血糖水平和胰岛素耐量。IRN治疗可减轻胎盘炎症。此外,irn处理组胎盘氧化应激得到缓解,导致胎盘功能改善,胎儿发育更健康。与未处理的GDM小鼠相比,irn处理组的后代出生体重更高。此外,IRN抑制NLRP3通路的激活。IRN通过NF-κB/NLRP3通路显著改善GDM的代谢和炎症参数,突出了其在控制GDM和改善母胎结局方面的潜在治疗益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isorhynchophylline Inhibits NLRP3 Inflammasome and Improves Gestational Diabetes.

This study aims to investigate the effects and underlying mechanisms of isorhynchophylline (IRN) on gestational diabetes mellitus (GDM). The db/+ mice were randomly divided into four groups: GDM, GDM + IRN (20 mg/kg), and GDM + IRN (40 mg/kg). Blood glucose and insulin tolerance were assessed using intraperitoneal glucose tolerance tests (IPGTTs) and intraperitoneal insulin tolerance tests (IPITTs) on gestational day 10. On gestational day 20, placental inflammation (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-1β), oxidative stress markers (malondialdehyde [MDA], SOD, glutathione peroxidase [GPx], and glutathione [GSH]), and nuclear factor-κB/NOD-like receptor protein 3 [NLRP3] inflammasome activity were measured using enzyme-linked immunosorbent assay (ELISA), immunoblotting, and biochemical assays. IRN significantly improved blood glucose levels and insulin tolerance in GDM mice. IRN treatment reduced placental inflammation. In addition, oxidative stress in the placenta was alleviated in the IRN-treated groups, leading to improved placental function and healthier fetal development. The birth weight of offspring was higher in the IRN-treated groups compared with untreated GDM mice. Furthermore, IRN inhibited the activation of the NLRP3 pathway. IRN significantly improves metabolic and inflammatory parameters in GDM through the NF-κB/NLRP3 pathway, highlighting its potential therapeutic benefits for managing GDM and improving maternal and fetal outcomes.

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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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