基于maldi - msi的方法表征大鼠肠组织中圆筒精子素的空间分布和脂质改变。

IF 4.7 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Antonio Casas-Rodríguez , Cristina María López-Vázquez , Remedios Guzmán-Guillén , Nahúm Ayala , Ana María Cameán , Angeles Jos , Eduardo Chicano-Gálvez
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引用次数: 0

摘要

全球变暖和水体富营养化正在推动蓝藻繁殖的增加,蓝藻会产生诸如圆柱形精子素(CYN)之类的毒素。该化合物具有多种毒性作用,在接触CYN后,其在体内的分布会发生变化,特别是在肝脏和肾脏等器官中,这表明其在关键组织中的生物蓄积潜力。基质辅助激光解吸/电离飞行时间质谱成像(MALDI-MSI)能够可视化各种分子的空间分布。本研究利用MALDI-MSI技术,建立并优化了一种检测CYN的新方法,并首次对暴露于该毒素(500 μg/kg体重)后0、2、4、6和24 h处死的大鼠肠道样品中CYN的定量时空分布进行了分析。此外,我们还评估了CYN对肠道脂质谱的影响。通过测量不同浓度(1-100 ppm)的模拟组织切片中的CYN,验证了该方法的线性度、灵敏度和精密度,使其成功应用于观察大鼠肠道中CYN的分布。结果显示,不同脂质家族的改变参与了炎症反应、氧化应激增加和对细胞膜完整性的进行性损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A MALDI-MSI-based approach to characterize the spatial distribution of cylindrospermopsin and lipid alterations in rat intestinal tissue
Global warming and eutrophication of water bodies are driving the increase in cyanobacterial blooms, which produce toxins such as cylindrospermopsin (CYN). This compound has multiple toxic effects, and following CYN exposure, its distribution in the body varies, particularly in organs such as the liver and kidneys, suggesting its potential for bioaccumulation in key tissues. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-MSI) enables visualization of the spatial distribution of a wide range of molecules. In this study, using MALDI-MSI, a new method was developed and optimized for the detection of CYN, and its quantitative spatiotemporal distribution was analyzed for the first time in intestinal samples from rats orally exposed to this toxin (500 μg/kg body weight) and sacrificed 0, 2, 4, 6 and 24 h after exposure. Furthermore, the impact of CYN on the intestinal lipid profile was evaluated. The method was validated in terms of linearity, sensitivity, and precision, measuring CYN in mimetic tissue sections at different concentrations (1–100 ppm), allowing its successful application to visualize CYN distribution in rat intestines. The results revealed alterations in different lipid families involved in the inflammatory response, increased oxidative stress, and progressive damage to the integrity of the cell membrane.
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来源期刊
CiteScore
7.70
自引率
3.90%
发文量
410
审稿时长
36 days
期刊介绍: Chemico-Biological Interactions publishes research reports and review articles that examine the molecular, cellular, and/or biochemical basis of toxicologically relevant outcomes. Special emphasis is placed on toxicological mechanisms associated with interactions between chemicals and biological systems. Outcomes may include all traditional endpoints caused by synthetic or naturally occurring chemicals, both in vivo and in vitro. Endpoints of interest include, but are not limited to carcinogenesis, mutagenesis, respiratory toxicology, neurotoxicology, reproductive and developmental toxicology, and immunotoxicology.
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