{"title":"MARVELD1 inhibits Neuro2a cell migration and tumorigenesis via regulating the transcriptional coactivators and protein methylation.","authors":"Weizhe Liu, Yucui Dong, Ruiying Guo, Dingyan Zhou, Yiping Qin, Yuetao Ma, Juanjuan Zhang, Aiying Li","doi":"10.1016/j.bulcan.2025.01.010","DOIUrl":null,"url":null,"abstract":"<p><p>Neuronal cell proliferation and migration play an important regulatory role in the development and tumorigenesis of the nervous system. MARVEL domain-containing protein 1 (MARVELD1) is a potential tumor suppressor gene whose function in nervous system diseases is unclear. This study aimed to analyze the function and molecular mechanism of MARVELD1 gene in Neuro2a cell migration and tumor growth. We found that MARVELD1 could inhibit the tumor development with low-expression of Ki67, high-expression of cleaved-caspase3 and strong signal TUNEL staining. Moreover, MARVELD1 suppressed migration and epithelial to mesenchymal transition process in Neuro2a cells. Mass spectrometry analysis indicated that MARVELD1 could bind to PPP1CB, PPP1CC and NRAS. The RNA-sequencing analysis showed that MARVELD1 regulated transcriptional coactivators and protein methylation. These genes ultimately affected the pathways related to cell migration. These data highlight the potential usefulness of MARVELD1 as a potential target for preventing nervous system tumor genesis and metastasis.</p>","PeriodicalId":93917,"journal":{"name":"Bulletin du cancer","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin du cancer","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.bulcan.2025.01.010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
神经元细胞的增殖和迁移在神经系统的发育和肿瘤发生中起着重要的调节作用。MARVEL domain-containing protein 1（MARVELD1）是一种潜在的肿瘤抑制基因，其在神经系统疾病中的功能尚不清楚。本研究旨在分析MARVELD1基因在Neuro2a细胞迁移和肿瘤生长中的功能和分子机制。我们发现，MARVELD1能抑制Ki67低表达、caspase3高表达和TUNEL染色强信号的肿瘤发生。此外，MARVELD1还能抑制Neuro2a细胞的迁移和上皮向间充质转化过程。质谱分析表明，MARVELD1能与PPP1CB、PPP1CC和NRAS结合。RNA 序列分析表明，MARVELD1 可调控转录辅激活因子和蛋白质甲基化。这些基因最终影响了与细胞迁移相关的通路。这些数据凸显了MARVELD1作为预防神经系统肿瘤发生和转移的潜在靶点的潜在作用。
MARVELD1 inhibits Neuro2a cell migration and tumorigenesis via regulating the transcriptional coactivators and protein methylation.
Neuronal cell proliferation and migration play an important regulatory role in the development and tumorigenesis of the nervous system. MARVEL domain-containing protein 1 (MARVELD1) is a potential tumor suppressor gene whose function in nervous system diseases is unclear. This study aimed to analyze the function and molecular mechanism of MARVELD1 gene in Neuro2a cell migration and tumor growth. We found that MARVELD1 could inhibit the tumor development with low-expression of Ki67, high-expression of cleaved-caspase3 and strong signal TUNEL staining. Moreover, MARVELD1 suppressed migration and epithelial to mesenchymal transition process in Neuro2a cells. Mass spectrometry analysis indicated that MARVELD1 could bind to PPP1CB, PPP1CC and NRAS. The RNA-sequencing analysis showed that MARVELD1 regulated transcriptional coactivators and protein methylation. These genes ultimately affected the pathways related to cell migration. These data highlight the potential usefulness of MARVELD1 as a potential target for preventing nervous system tumor genesis and metastasis.
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