m6a修饰的LINC02418在结直肠癌中通过与YBX1和IGF2BP1相互作用诱导CTNNB1的转录和转录后修饰。

IF 6.1 2区 生物学 Q1 CELL BIOLOGY
Hao Zhang, Ye Han, Chengwei Wu, Siying Wang, Mingquan Chen, Qian Xu, Hong Wei, Xianli Zhou, Guiyu Wang
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引用次数: 0

摘要

结直肠癌(Colorectal cancer, CRC)是危害人类健康的重大疾病,但其分子发病机制尚不清楚。在此,我们探讨了LINC02418在结直肠癌进展中的功能作用。通过体外和体内实验确定LINC02418在结直肠癌中的功能。采用实时荧光定量PCR (qPCR)、western blot、荧光素酶报告基因法、甲基化RNA免疫沉淀(MeRIP)法、RNA拉下、RNA免疫沉淀(RIP)法和染色质免疫沉淀(ChIP)法探讨LINC02418在结直肠癌中的分子机制。结果显示,LINC02418在结直肠癌组织中表达上调,且LINC02418的高表达与结直肠癌患者的不利生存有关。此外,在体外和体内,敲低LINC02418的表达可抑制CRC细胞的增殖和转移。在机制上,我们发现mettl3介导的m6A修饰诱导了CRC中LINC02418的异常表达。LINC02418可以与YBX1相互作用,增强YBX1与CTNNB1启动子的dna结合能力,从而激活CTNNB1的转录。在转录后阶段,LINC02418还可以通过促进IGF2BP1蛋白与CTNNB1 mRNA的相互作用来增强CTNNB1的稳定性。此外,YBX1蛋白可通过转录增强LINC02418的表达。总的来说,本研究揭示了LINC02418在结直肠癌中的一种新的致癌机制,并且LINC02418可能成为结直肠癌治疗的一种新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
m6A-modified LINC02418 induces transcriptional and post-transcriptional modification of CTNNB1 via interacting with YBX1 and IGF2BP1 in colorectal cancer.

Colorectal cancer (CRC) represents a significant menace to human health, but its molecular pathogenesis remains unclear. Herein, we explored the functional role of LINC02418 in CRC progression. The function of LINC02418 in CRC was determined through vitro and in vivo experiments. The molecular mechanism of LINC02418 in CRC was explored by quantitative real-time PCR (qPCR) analyses, western blot, luciferase reporter assay, methylated RNA immunoprecipitation (MeRIP) assay, RNA pull-down, RNA immunoprecipitation (RIP) assay and chromatin immunoprecipitation (ChIP) assay. The results revealed that LINC02418 expression was upregulated in CRC tissues and the high expression of LINC02418 was related to unfavorable survival of CRC patients. Besides, knockdown of LINC02418 expression resulted in the inhibition of proliferation and metastasis of CRC cells in vitro and in vivo. Mechanistically, we found METTL3-mediated m6A modification induced the aberrant expression of LINC02418 in CRC. LINC02418 could interact with YBX1 and enhance YBX1 DNA-binding ability to the CTNNB1 promoter, resulting in transcriptional activation of CTNNB1. In the post-transcriptional stage, LINC02418 could also enhance CTNNB1 stability by promoting the interaction between IGF2BP1 protein and CTNNB1 mRNA. What is more, LINC02418 expression could be transcriptionally enhanced by YBX1 protein. Collectively, this study unveils a novel oncogenic mechanism for LINC02418 in CRC and the LINC02418 might be a novel therapeutic target in CRC treatment.

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来源期刊
Cell Death Discovery
Cell Death Discovery Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
8.30
自引率
1.40%
发文量
468
审稿时长
9 weeks
期刊介绍: Cell Death Discovery is a multidisciplinary, international, online-only, open access journal, dedicated to publishing research at the intersection of medicine with biochemistry, pharmacology, immunology, cell biology and cell death, provided it is scientifically sound. The unrestricted access to research findings in Cell Death Discovery will foster a dynamic and highly productive dialogue between basic scientists and clinicians, as well as researchers in industry with a focus on cancer, neurobiology and inflammation research. As an official journal of the Cell Death Differentiation Association (ADMC), Cell Death Discovery will build upon the success of Cell Death & Differentiation and Cell Death & Disease in publishing important peer-reviewed original research, timely reviews and editorial commentary. Cell Death Discovery is committed to increasing the reproducibility of research. To this end, in conjunction with its sister journals Cell Death & Differentiation and Cell Death & Disease, Cell Death Discovery provides a unique forum for scientists as well as clinicians and members of the pharmaceutical and biotechnical industry. It is committed to the rapid publication of high quality original papers that relate to these subjects, together with topical, usually solicited, reviews, editorial correspondence and occasional commentaries on controversial and scientifically informative issues.
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