IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Wen-Bo Gao, Shu-Ting Xu, Yong-Jie Yan, Cheng Yang, Xiu-Ping Yan
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引用次数: 0

摘要

氨基酸是功能食品中常用的营养强化物质,其手性构型是决定食品功能的重要因素。食品分析迫切需要快速的手性筛选方法,但受限于长时间的手性分离和基质干扰。在本研究中,我们展示了一种动力学方法,该方法与热辅助纸喷雾电离质谱联用,无需样品预处理即可直接测定复杂食品基质中多种 d/l- 氨基酸的对映体过量(ee)。该动力学方法选择了 3-(2-萘基)-l-丙氨酸作为新的手性参考配体,以实现高效的手性区分(d/l-苯丙氨酸的区分度为 8.7,d/l-酪氨酸的区分度为 10.2)。为了便于对映体纯度的测定,还为喷纸电离质谱法开发了一个额外的热辅助装置。所开发的方法可在 30 秒内同时快速测定多种手性氨基酸(d/l-苯丙氨酸和 d/l-酪氨酸)的对映体纯度。在未知氨基酸浓度为 10 μM 至 600 μM 的情况下,ee 的定量线性良好(苯丙氨酸的 R2 = 0.9996,酪氨酸的 R2 = 0.9995)。所建立的方法成功地应用于功能性胶囊和饮料中多种手性氨基酸对映体纯度的测定,在高效的对映体相关食品安全筛查和营养分析中显示出巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetic Method Coupled with Thermal-Assisted Paper Spray Ionization Mass Spectrometry for Direct Determination of Enantiomeric Excess of Multiple d/l-Amino Acids in Functional Foods.

Amino acids are commonly used as nutritional fortification substances in functional foods, and their chiral configuration is an important determinant of food function. Rapid chiral screening methods are urgently needed in food analysis but are limited by the long-time chiral separation and matrix interference. In this study, we show a kinetic method coupled to thermal-assisted paper spray ionization mass spectrometry for direct determination of enantiomeric excess (ee) of multiple d/l-amino acids in complex food matrixes without sample pretreatment. 3-(2-Naphthyl)-l-alanine was selected as a new chiral reference ligand for the kinetic method to achieve efficient chiral differentiation (discrimination degree is 8.7 for d/l-phenylalanine and 10.2 for d/l-tyrosine). An additional thermal-auxiliary device was developed for paper spray ionization mass spectrometry to facilitate the enantiomeric purity determination. The developed method allowed a rapid simultaneous enantiomeric purity determination of multiple chiral amino acids (d/l-phenylalanine and d/l-tyrosine) within 30 s. Good linearities were achieved for the quantitation of ee (R2 = 0.9996 for phenylalanine and 0.9995 for tyrosine) with unknown amino acid concentrations ranging from 10 μM to 600 μM. The developed method was successfully applied for the enantiomeric purity determination of multiple chiral amino acids in functional capsules and beverages and showed great potential for efficient enantiomer-related food safety screening and nutrition analysis.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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