MALDI成像对脑PET示踪剂空间分布的影响。

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Isabeau Vermeulen, Michiel Vandenbosch, Delphine Viot, Joel Mercier, Diego Asensio-Wandosell Cabañas, Pilar Martinez-Martinez, Patrick Barton, Ron M A Heeren, Berta Cillero-Pastor
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引用次数: 0

摘要

评估正电子发射断层扫描(PET)示踪剂在其开发过程中的组织分布通常涉及放射自显影技术,其中放射性化合物在临床前开发阶段用于组织的离体可视化和量化。质谱成像(MSI)提供了一个潜在的替代方案,在不需要放射性的情况下提供空间信息,具有相似的空间分辨率。本研究旨在优化MSI样品制备方案,以体外评估PET示踪剂候选物,重点研究两种化合物:UCB-J和UCB2400。我们测试了不同的基质,并介绍了洗涤步骤,以提高PET示踪剂的检测。制备组织匀浆,建立定量校准曲线。在MSI样品制备方案中加入洗涤步骤增强了两种PET示踪剂的信号。我们的研究结果突出了MSI作为评估PET示踪剂分布的成本效益和有效方法的潜力。本文提供的优化方法可以提供一种增强信号和最小化离子抑制效应的协议,这对于未来MSI研究中PET示踪剂的评估具有价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spatial Distribution of Brain PET Tracers by MALDI Imaging.

Evaluating tissue distribution of Positron Emission Tomography (PET) tracers during their development conventionally involves autoradiography techniques, where radioactive compounds are used for ex vivo visualization and quantification in tissues during preclinical development stages. Mass Spectrometry Imaging (MSI) offers a potential alternative, providing spatial information without the need for radioactivity with a similar spatial resolution. This study aimed to optimize a MSI sample preparation protocol for assessing PET tracer candidates ex vivo with a focus on two compounds: UCB-J and UCB2400. We tested different matrices and introduced washing steps to improve PET tracer detection. Tissue homogenates were prepared to construct calibration curves for quantification. The incorporation of a washing step into the MSI sample preparation protocol enhanced the signal of both PET tracers. Our findings highlight MSI's potential as a cost-effective and efficient method for the evaluation of PET tracer distribution. The optimized approach offered here can provide a protocol that enhances the signal and minimizes ion suppression effect, which can be valuable for future evaluation of PET tracers in MSI studies.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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