小麦黄锈病的抗性基因:在PI348764中发现了可能的新抗病基因IARI276和Yr5。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2025-01-01 DOI:10.1139/gen-2024-0171
Lovely Arya, Malavika N Shal, M Niranjana, S K Jha, Madhu Patial, M S Saharan, N Mallick, K Raghunandan, A Priyanka, D P Walia, S M S Tomar, Vinod
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引用次数: 0

摘要

由小麦条锈病引起的黄条锈病是影响全球小麦生产的主要生物胁迫。由小麦PI348764和IARI276获得的侵染系在幼苗期和成株期均表现出较高的抗黄锈性。位于2B染色体上的Yr5基因是此前在褐霉基因库中唯一被发现的抗条锈病基因。通过使用与Yr5基因相关的标记对亲本和渐渗材料进行基因分型,我们证明PI348764可能携带Yr5基因,而IARI276似乎不携带Yr5基因。通过多种田间试验的成株抗性和幼苗抗性筛选、F5家族的大量分离分析(BSA)和小麦育种者的35K阵列基因分型等综合方法,我们发现YrIARI276是一种新的抗条锈病基因,其染色体位置可能位于1BL、1DL、5AL或7BL上。此外,遗传分析表明,YrIARI276对单一显性基因的孟德尔比率具有良好的拟合性。由于该基因与Yr5不同,并且染色体位置与先前报道的Yr基因不同,因此它将有助于在抗病育种计划中提高Yr基因库的多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Resistance genes against yellow rust pathogen in Triticum spelta: a possible new Yr resistance gene in accession IARI276 and Yr5 presence confirmed in PI348764.

Yellow/stripe rust caused by Puccinia striiformis f. sp. tritici is a major biotic stress in global wheat production. Introgression lines derived from the Triticum spelta accessions PI348764 and IARI276 showed high levels of yellow rust resistance at seedling and adult plant stage. The Yr5 gene located on 2B chromosome was previously the only stripe rust resistance gene described in T. spelta gene pool. By genotyping parental and introgressed material with markers linked with the Yr5 gene, we demonstrate that PI348764 likely carries Yr5, and that it appears to be absent from IARI276. By employing a combination of methods, including screening for adult plant resistance and seedling resistance at multiple field trials, bulked segregant analysis (BSA) on F5 families, and genotyping using wheat Breeders' 35K array, we show that YrIARI276 is a novel stripe rust resistance gene with putative chromosomal locations on 1BL, 1DL, 5AL, or 7BL. Furthermore, genetic analysis revealed that YrIARI276 showed a goodness of fit to Mendelian ratios for a single dominant gene. As the gene is distinct from Yr5 and the chromosomal location is unique from earlier reported Yr genes, it will be useful in improving diversity of Yr gene repertoire in disease resistance breeding programmes.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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