金黄色葡萄球菌感染时胞浆mtDNA释放分析。

4区 生物学 Q4 Biochemistry, Genetics and Molecular Biology
Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-11-04 DOI:10.1016/bs.mcb.2024.09.003
Caterina Licini, Gloria D'Achille, Nada Dhaouadi, Ilaria Nunzi, Fabio Marcheggiani, Matteo Fabbri, Monica Mattioli-Belmonte, Gianluca Morroni, Saverio Marchi
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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌(MRSA)是主要的人类病原体之一,引起皮肤伤口的严重感染。MRSA感染主要通过线粒体介导途径引发细胞反应,导致线粒体外膜通透,线粒体内膜挤压进入细胞质,线粒体DNA (mtDNA)溢出进入细胞质。由于mtDNA的特性,细胞将排出的细胞质mtDNA (cmtDNA)识别为“非自身”,并触发级联事件,如炎症小体的激活。在这里,我们详细介绍了一种检测和测量永生化角质形成细胞(HaCaT细胞)感染MRSA后不同时间点mtDNA释放到细胞质中的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of cytosolic mtDNA release during Staphylococcus aureus infection.

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the principal human pathogens, causing severe infections in skin wounds. MRSA infection triggers a cell response mainly by mitochondrial-mediated pathway, resulting in mitochondrial outer membrane permeabilization, extrusion of the mitochondrial inner membrane into the cytoplasm, and then spillage of mitochondrial DNA (mtDNA) into the cytoplasm. The cell recognizes the discharged cytosolic mtDNA (cmtDNA) as "not-itself" because of mtDNA properties and triggers cascade events, such as the activation of inflammasomes. Here, we detail a method to detect and measure the mtDNA release into the cytoplasm in immortalized keratinocytes (HaCaT cells), after the infection with MRSA at different time points after the infection.

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来源期刊
Methods in cell biology
Methods in cell biology 生物-细胞生物学
CiteScore
3.10
自引率
0.00%
发文量
125
审稿时长
3 months
期刊介绍: For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
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