利用微生物诱导碳酸盐沉淀（MICP）进行生物布线，改善可能液化的颗粒土的物理和机械性能

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2025-02-24 DOI:10.1016/j.mex.2025.103246

Nur Ayu Diana , Ria Asih Aryani Soemitro , Januarti Jaya Ekaputri , Trihanyndio Rendy Satrya , Dwa Desa Warnana

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引用次数: 0

摘要

生物灌浆是一种利用微生物和机械技术增强土壤性质的方法，在土壤改良方面显示出巨大的潜力。大多数研究集中在实验室的小砂柱上，但最近的测试使用了0.5米的塑料盒，里面装满了用微生物和粉煤灰稳定的沙子。实验进行了30多天，采用微生物液注射和输液的方法，维持地下水水位以模拟现场条件。通过无侧限抗压强度（UCS）试验分析了提取样品的力学性能。研究人员还评估了碳酸钙的分布和抗剪强度。结果表明，含水饱和度对垂向应力（qu）有显著影响，而UCS与不同碳酸钙含量砂的渗透率相关。在这一过程中使用的一种有弹性的细菌萨芬芽孢杆菌可以承受极端条件。完成任务后，它进入休眠状态，并在需要时重新激活。这种细菌通过将钙与酶结合产生碳酸钙，从而使土壤颗粒粘合，增强强度和稳定性。•在微生物和天然土壤上测试酶；•使用输液进行滴管工具的安装设置；使用UCS稳定后进行土壤阻力测试

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Biogrouting with microbial-induced carbonate precipitation (MICP) for improving the physical and mechanical properties of granular soils potential liquefaction

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Biogrouting with microbial-induced carbonate precipitation (MICP) for improving the physical and mechanical properties of granular soils potential liquefaction

Biogrouting, a method to enhance soil properties using microorganisms and mechanical techniques, has shown great potential for soil improvement. Most studies focus on small sand columns in labs, but recent tests used 0.5 m plastic boxes filled with sand stabilized with microorganisms and fly ash. The experiments, conducted over 30 days, applied injection and infusion methods with microbial fluids, maintaining groundwater levels to simulate field conditions. Mechanical properties were analyzed through unconfined compressive strength (UCS) tests on extracted samples. Researchers also assessed calcium carbonate distribution and shear strength. Results showed water saturation significantly influenced vertical stress (qu), while UCS correlated with the permeability of sand containing varying calcium carbonate levels. Bacillus safensis, a resilient bacterium used in this process, can withstand extreme conditions. After completing its task, it enters a dormant state and reactivates when needed. The bacteria produce calcium carbonate by binding calcium with enzymes, which cements soil particles, enhancing strength and stability.