糖尿病相关牙周炎的非手术治疗对免疫/炎症和氧化应激生物标志物的影响：一项初步研究

IF 2.9 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Journal of diabetes and its complications Pub Date : 2025-03-07 DOI:10.1016/j.jdiacomp.2025.108999

Steffany Bernardo Oliveira , Ana Luíza Pereira Assunção Silveira , Yeon Jung Kim , Jônatas Bussador do Amaral , André Luis Lacerda Bachi , Margareth Afonso Torres , Karen Melissa Gonçalves Oliveira , Daniela Alves de Abreu , Leonardo Diniz Resende , Débora Pallos , Carolina Nunes França

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引用次数: 0

摘要

目的探讨非手术牙周治疗对糖尿病患者和非糖尿病患者牙周细胞因子、sIgA、抗菌肽、氧化和抗氧化剂水平的影响。方法一项病例对照研究，纳入了伴有或不伴有糖尿病的牙周炎患者（n = 45）。分别用LUMINEX和ELISA法测定龈沟液（GCF）和唾液中细胞因子的浓度。在牙周炎治疗结束前（T1）和治疗结束后1个月（T1），用比色法测定唾液溶菌酶、抗菌素（LL-37）、总抗氧化能力（TAC）和总过氧化物水平。结果各组间及T0、T1间GCF细胞因子浓度差异无统计学意义。与T0相比，糖尿病组T1时唾液溶菌酶和IL-10浓度显著降低（p = 0.0260和p = 0.0034），而TNF-α浓度升高（p = 0.0443）。非糖尿病组T1时唾液中TNF-α浓度在T0时下降（p = 0.0313）。与非糖尿病组相比，糖尿病组唾液中TNF-α浓度在T1时升高（p = 0.0008）。结论非手术牙周治疗后，非糖尿病组唾液炎症状态有明显改善，而糖尿病组无明显改善。

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Effect of non-surgical treatment in diabetes-associated periodontitis on immune/inflammatory and oxidative stress biomarkers: A pilot study

Aims

To investigate the effects of non-surgical periodontal treatment on the levels of cytokines, sIgA, antimicrobial peptides, oxidative and antioxidative agents in comparison between patients with and without diabetes.

Methods

A case-control study that included patients (n = 45) with periodontitis who have or do not have diabetes. Cytokine concentrations in crevicular gingival fluid (GCF) and saliva were determined using LUMINEX and ELISA assays, respectively. Salivary levels of lysozyme, cathelicidin (LL-37), total antioxidant capacity (TAC) and total peroxide were determined colorimetrically before (T0) and one month after completion of periodontitis treatment (T1).

Results

There were no significant differences in the concentrations of cytokines in GCF between the groups and T0 and T1. Salivary concentrations of lysozyme and IL-10 were significantly reduced in T1 compared to T0 in the diabetes group (p = 0.0260 and p = 0.0034, respectively), whilst TNF-α concentration was higher in T1 (p = 0.0443). The salivary concentrations of TNF-α observed in the non-diabetes group at T1 decreased at T0 (p = 0.0313). The salivary concentration of TNF-α was increased in the diabetes group compared to the non-diabetes group at T1 (p = 0.0008).

Conclusions

An improvement in salivary inflammatory status was observed in the group without diabetes, which was not found in the group with diabetes after non-surgical periodontal treatment.

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来源期刊

Journal of diabetes and its complications 医学-内分泌学与代谢

CiteScore

5.90

自引率

3.30%

发文量

153

审稿时长

16 days

期刊介绍： Journal of Diabetes and Its Complications (JDC) is a journal for health care practitioners and researchers, that publishes original research about the pathogenesis, diagnosis and management of diabetes mellitus and its complications. JDC also publishes articles on physiological and molecular aspects of glucose homeostasis. The primary purpose of JDC is to act as a source of information usable by diabetes practitioners and researchers to increase their knowledge about mechanisms of diabetes and complications development, and promote better management of people with diabetes who are at risk for those complications. Manuscripts submitted to JDC can report any aspect of basic, translational or clinical research as well as epidemiology. Topics can range broadly from early prediabetes to late-stage complicated diabetes. Topics relevant to basic/translational reports include pancreatic islet dysfunction and insulin resistance, altered adipose tissue function in diabetes, altered neuronal control of glucose homeostasis and mechanisms of drug action. Topics relevant to diabetic complications include diabetic retinopathy, neuropathy and nephropathy; peripheral vascular disease and coronary heart disease; gastrointestinal disorders, renal failure and impotence; and hypertension and hyperlipidemia.