女贞草和女贞草中的Specnuezhenide和ecliptasaponin A通过靶向ESR1改善卵巢早衰

IF 3 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Jia Xu , Lei Lei , Ping Li , Zi-Chun Huang , Ying Meng , Bing He , Ji-Lin Kuang
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引用次数: 0

摘要

本研究旨在探讨女贞子和黄花草对卵巢早衰(POF)的作用及其机制。在使用环磷酰胺(CTX)构建的POF小鼠模型中,女贞子和黄花草可提高卵巢指数和雌二醇(E2)水平,降低卵巢活力和促卵泡激素(FSH)水平。女贞、茱萸均能减轻POF小鼠卵巢病理损伤,促进卵巢CD31和血管内皮生长因子A (VEGFA)的表达。通过高效液相色谱-质谱(HPLC-MS)和网络药理学方法,鉴定了女贞子和女贞子抗pof的关键成分为Specnuezhenide和ecliptasaponin A。与这些成分相关的重要靶点是雌激素受体(ESR) 1。分子对接和体外实验表明,Specnuezhenide和ecliptasaponin A均能与ESR蛋白结合;抑制ESR1抑制Specnuezhenide和ecliptasaponin A对ctx诱导的POF细胞的抗凋亡作用。综上所述,女贞子和黄柏中缓解POF的关键成分是Specnuezhenide和ecliptasaponin A,它们通过上调ESR1来改善POF。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Specnuezhenide and ecliptasaponin A from Ligustrum lucidum Ait and Ecliptae Herba improved premature ovarian failure by targeting the ESR1
This study was designed to investigate the role of Ligustrum lucidum Ait and Ecliptae Herba on premature ovarian failure (POF) and the underlying mechanisms. In the POF mouse model constructed using cyclophosphamide (CTX), Ligustrum lucidum Ait and Ecliptae Herba increased ovarian index and estradiol (E2) levels and curtailed motility and follicle-stimulating hormone (FSH). Ligustrum lucidum Ait and Ecliptae Herba alleviated ovarian pathological damage in POF mice and promoted the expression of ovarian CD31 and Vascular Endothelial Growth Factor A (VEGFA). Through high-performance liquid chromatography-mass spectrometry (HPLC-MS) and network pharmacology, Specnuezhenide and ecliptasaponin A were identified as the key components of Ligustrum lucidum Ait and Ecliptae Herba in anti-POF action. The important target associated with these components is Estrogen Receptor (ESR) 1. Molecular docking and in vitro experiments showed that Specnuezhenide and ecliptasaponin A can both bind to the ESR protein; knocking down ESR1 inhibited the anti-apoptotic effect of Specnuezhenide and ecliptasaponin A on CTX-induced POF cells. In conclusion, the key components of Ligustrum lucidum Ait and Ecliptae Herba that alleviate POF are Specnuezhenide and ecliptasaponin A, which improve the condition by upregulating ESR1.
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来源期刊
CiteScore
6.20
自引率
2.90%
发文量
104
审稿时长
31 days
期刊介绍: Journal of Pharmacological Sciences (JPS) is an international open access journal intended for the advancement of pharmacological sciences in the world. The Journal welcomes submissions in all fields of experimental and clinical pharmacology, including neuroscience, and biochemical, cellular, and molecular pharmacology for publication as Reviews, Full Papers or Short Communications. Short Communications are short research article intended to provide novel and exciting pharmacological findings. Manuscripts concerning descriptive case reports, pharmacokinetic and pharmacodynamic studies without pharmacological mechanism and dose-response determinations are not acceptable and will be rejected without peer review. The ethnopharmacological studies are also out of the scope of this journal. Furthermore, JPS does not publish work on the actions of biological extracts unknown chemical composition.
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