Wenxin Hu, Jie Wei, Sen Zheng, Guan Jiang, Bei Zhang, Zhen Liang
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Hela cells were treated in vitro with 5-ALA-UMNs, and the cellular uptake of the photosensitizer was observed using inverted fluorescence microscopy. Cell viability was determined by the CCK-8 assay to identify the optimal drug concentration. Additionally, the anti-tumor efficacy of 5-ALA-UMNs, induced via photodynamic therapy, was evaluated by Live-Dead staining and flow cytometry.</p><p><strong>Results: </strong>The microneedles exhibited a uniform quadrangular pyramidal shape, orderly arrangement, intact needle tips, and robust mechanical strength. Inverted fluorescence microscopy confirmed the successful uptake of the photosensitizer by Hela cells, which enzymatically converted it to the fluorescent compound protoporphyrin IX. CCK-8 assays demonstrated that 5-ALA-UMNs displayed favorable cytocompatibility and safety. Live-dead staining revealed Hela cell survival rates as follows: 99.55% in the control group, 99.37% in the control microneedle group, 99.41% in the 5-ALA-UMNs group without light exposure, and 57.35% in the 5-ALA-UMNs group with light exposure (all p < 0.05). Flow cytometry results corroborated the live-dead staining findings, confirming the cytotoxic effect of 5-ALA-UMNs on tumor cells.</p><p><strong>Conclusion: </strong>These results indicate that 5-ALA-UMNs hold promise as a tumor-targeting therapeutic.</p>","PeriodicalId":7934,"journal":{"name":"Anti-cancer agents in medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ulvan Microneedles Loaded with Photosensitizer 5-aminolevulinic Acid Inhibits Human Cervical Cancer HeLa cells In vitro.\",\"authors\":\"Wenxin Hu, Jie Wei, Sen Zheng, Guan Jiang, Bei Zhang, Zhen Liang\",\"doi\":\"10.2174/0118715206358815250224043946\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cervical cancer encompasses highly invasive and metastatic malignant tumors with poor prognoses. 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引用次数: 0
摘要
背景：宫颈癌是一种预后不良的高度侵袭性和转移性恶性肿瘤。近年来，微针作为一种新型的非侵入性给药方法，在肿瘤治疗中具有独特的优势，引起了人们的广泛关注。目的：建立以紫丁香素为基础包封光敏剂5-氨基乙酰丙酸（5-ALA-UMNs）的微针给药系统，研究其对人宫颈癌Hela细胞生长的抑制作用。方法：采用两步铸造技术制备5- ala - umn和对照微针（不含光敏剂）。评估微针的形态、穿刺性能和机械强度。用5-ALA-UMNs体外处理Hela细胞，倒置荧光显微镜观察光敏剂的细胞摄取情况。采用CCK-8法测定细胞活力，确定最佳药物浓度。此外，通过活死染色和流式细胞术评估光动力诱导的5-ALA-UMNs的抗肿瘤效果。结果：微针呈均匀的四边形锥体，排列有序，针尖完整，机械强度强。倒置荧光显微镜证实Hela细胞成功地吸收了光敏剂，并将其酶促转化为荧光化合物原卟啉IX。CCK-8实验表明，5- ala - umn具有良好的细胞相容性和安全性。活死染色显示，对照组Hela细胞存活率为99.55%，对照组微针组为99.37%，未光照5-ALA-UMNs组为99.41%，光照5-ALA-UMNs组为57.35% （p < 0.05）。流式细胞术结果证实了活死染色结果，证实了5-ALA-UMNs对肿瘤细胞的细胞毒作用。结论：这些结果表明5- ala - umn有望成为肿瘤靶向治疗药物。
Ulvan Microneedles Loaded with Photosensitizer 5-aminolevulinic Acid Inhibits Human Cervical Cancer HeLa cells In vitro.
Background: Cervical cancer encompasses highly invasive and metastatic malignant tumors with poor prognoses. Recently, microneedles have gained significant attention as a novel, non-invasive drug delivery method, offering unique advantages in tumor treatment.
Objective: This study aims to develop an ulvan-based microneedle delivery system encapsulating the photosensitizer 5-aminolevulinic acid (5-ALA-UMNs) and to investigate its inhibitory effects on the growth of human cervical cancer Hela cells.
Methods: The 5-ALA-UMNs and control microneedles (without photosensitizer) were fabricated using a twostep casting technique. The microneedles' morphology, puncture performance, and mechanical strength were assessed. Hela cells were treated in vitro with 5-ALA-UMNs, and the cellular uptake of the photosensitizer was observed using inverted fluorescence microscopy. Cell viability was determined by the CCK-8 assay to identify the optimal drug concentration. Additionally, the anti-tumor efficacy of 5-ALA-UMNs, induced via photodynamic therapy, was evaluated by Live-Dead staining and flow cytometry.
Results: The microneedles exhibited a uniform quadrangular pyramidal shape, orderly arrangement, intact needle tips, and robust mechanical strength. Inverted fluorescence microscopy confirmed the successful uptake of the photosensitizer by Hela cells, which enzymatically converted it to the fluorescent compound protoporphyrin IX. CCK-8 assays demonstrated that 5-ALA-UMNs displayed favorable cytocompatibility and safety. Live-dead staining revealed Hela cell survival rates as follows: 99.55% in the control group, 99.37% in the control microneedle group, 99.41% in the 5-ALA-UMNs group without light exposure, and 57.35% in the 5-ALA-UMNs group with light exposure (all p < 0.05). Flow cytometry results corroborated the live-dead staining findings, confirming the cytotoxic effect of 5-ALA-UMNs on tumor cells.
Conclusion: These results indicate that 5-ALA-UMNs hold promise as a tumor-targeting therapeutic.
期刊介绍：
Formerly: Current Medicinal Chemistry - Anti-Cancer Agents.
Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents.
Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication.
Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.
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