为资源贫乏的昆虫设计和验证一种低成本的糖饲料。

Gates Open Research Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI:10.12688/gatesopenres.16314.1
Zachary Thomas Stavrou-Dowd, Clair Rose, Álvaro Acosta-Serrano, Lee Rafuse Haines
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引用次数: 0

摘要

背景:昆虫中杀虫剂抗性的出现促使研究人员开发新的控制工具,以便在减少疾病传播方面取得的历史性成果不会丧失。有吸引力的靶向糖饵(ATSBs)是一种被广泛试验的病媒控制工具,用于瞄准以植物糖和血液为食的昆虫。我们设计了一种田间友好、经济且更环保的喂糖器,用于维持蚊子种群和筛选潜在的ATSB候选物。方法:在甘比亚按蚊的糖液中同时添加三种水溶性药用和食用染料(诱色红、荧光素和酒黄),以识别从喂食器中摄取糖的昆虫。为了测试喂食器释放有毒物质的效果,我们评估了使用硼酸杀死蚊子的效果,硼酸在摄入后可以杀死雄性和雌性蚊子。使用与实地工作相兼容的显微镜技术,包括使用手机相机,我们记录了连续喂食染色糖溶液后染料对蚊子的功效和组织特异性。结果:简易的投糖器是测定ATSB各成分对冈比亚按蚊药效的一种经济可行的方法。诱惑红AC是首选染料,因为它对蚊子的毒性低,并且可以让研究人员快速看到腹部吸收的糖餐。喂食1%的荧光素染料,而不是0.1%的荧光素染料,超过五天会引起全身的染料分布,当用手持式黑光手电筒(395-400nm发射)检查时,蚊子的翼静脉、触角和腿发出明亮的荧光。讨论:开发一种可负担得起的糖饲料,以维持昆虫饲养的昆虫,并测试ATSB候选物的功效,包括设计一种染料标记的糖诱饵站,该站具有低毒、可重复使用和易于构建,使用在低资源环境(如野外站)中可用的组件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Design and validation of a low-cost sugar-feeder for resource-poor insectaries.

Background: The emergence of insecticide resistance in insects has led researchers to develop new control tools so that historic gains made in reducing disease transmission are not lost. Attractive targeted sugar baits (ATSBs) are a vector control tool being widely trialled to target insects that feed on plant sugars and blood. We designed a field-friendly, economical and more environmentally responsible sugar feeder for maintaining mosquito colonies and screening potential ATSB candidates.

Methods: We simultaneously tested, in both male and female Anopheles gambiae mosquitoes, the effect of adding three water-soluble medical and food dyes (Allura Red, fluorescein and tartrazine) to the sugar solution to identify those insects that had ingested sugar from the feeder. To test feeder efficacy to deliver a toxic substance, we assessed the killing using boric acid, which kills both male and female mosquitoes when ingested. Using microscopy techniques compatible with fieldwork, including the use of a mobile phone camera, we documented the efficacy and tissue specificity of the dyes on mosquitoes after they were continuously fed dyed sugar solutions.

Results: The easy-to-construct sugar feeder is an economical option for testing the efficacy of ATSB components on Anopheles gambiae mosquitoes . Allura Red AC was the preferred dye as it has low toxicity to mosquitoes and allows the researcher to quickly visualise the imbibed sugar meal within the abdomen. Feeding 1% fluorescein dye, but not 0.1%, for longer than five days induced systemic dye distribution, where the mosquito's wing veins, antennae and legs brightly fluoresced when examined by a handheld black light torch (395-400nm emission).

Discussion: Developing an affordable sugar feeder to maintain insectary-reared insects and test the efficacy of ATSB candidates involves designing a dye-labelled sugar bait station that is of low-toxicity, reusable and easy to construct using components available in low resource settings such as field stations.

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来源期刊
Gates Open Research
Gates Open Research Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
90
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