Niemann-Pick c1样1蛋白在胃癌中的表达。

IF 1.6 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2025-03-01 DOI:10.21873/anticanres.17484

Takeharu Imai, Manabu Futamura, Ryutaro Mori, Itaru Yasufuku, Toshiyuki Tanahashi, Chiemi Saigo, Tamotsu Takeuchi, Nobuhisa Matsuhashi

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引用次数: 0

摘要

背景/目的：5-氟尿嘧啶（5-FU）在胃癌（GC）的治疗中发挥着重要作用，克服对该药物的耐药仍然是一个关键的挑战。本研究旨在鉴定GC对5-FU耐药的相关基因。材料和方法：分析5-FU耐药MKN-45/F2R细胞在5-FU处理前后（24和72 h）与亲本MKN-45细胞的基因表达水平。在持续过表达的基因中，进一步研究高表达基因在GC组织中的耐药作用和表达模式。结果：NPC1L1，一种假设影响5-FU耐药性的蛋白质，在测试细胞中没有表现出与耐药性相关的作用。随后在胃细胞中研究了其表达。在胃、空肠、十二指肠和肝脏中检测到NPC1L1，在一些GC样品中mRNA水平升高。95例胃癌患者的免疫染色显示39例（41.0%）NPC1L1表达升高≥10%。npc1l1阳性肿瘤患者预后较阴性肿瘤患者差。结论：NPC1L1在正常胃组织和胃癌组织中均有表达，侵袭部位表达水平升高与预后不良相关。

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Expression of the Niemann-Pick C1-like 1 Protein in Gastric Cancer.

Background/aim: 5-Fluorouracil (5-FU) plays a major role in the treatment of gastric cancer (GC), and overcoming resistance to this drug remains a critical challenge. This study aimed to identify genes associated with resistance to 5-FU in GC.

Materials and methods: Gene expression levels were analyzed in 5-FU-resistant MKN-45/F2R cells compared to the parental MKN-45 strain before and after 5-FU treatment (24 and 72 h). Among the consistently over-expressed genes, those with high expression were further investigated for their roles in resistance and expression patterns in GC tissues.

Results: NPC1L1, a protein hypothesized to influence 5-FU resistance, did not exhibit resistance-related effects in tested cells. Its expression was subsequently studied in gastric cells. NPC1L1 was detected in the stomach, jejunum, duodenum, and liver, with increased mRNA levels in several GC samples. Immunostaining of samples from 95 GC patients revealed that 39 (41.0%) exhibited ≥10% higher NPC1L1 expression. Patients with NPC1L1-positive tumors had poorer prognoses than those with negative tumors.

Conclusion: NPC1L1 is expressed in both normal gastric tissues and GC tissues, with elevated levels at invasive sites associated with poor prognosis.

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.