基于生物信息学的儿童哮喘细胞因子-细胞因子受体相关基因筛选。

IF 1.5 4区生物学 Q4 CELL BIOLOGY

Integrative Biology Pub Date : 2025-01-08 DOI:10.1093/intbio/zyaf002

Caiwen Wang, Zhimei Liu, Xiaoting Ren, Yiquan Li, Liping Sun

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引用次数: 0

摘要

目的：深入了解儿童哮喘的分子机制和潜在靶点，对开发有效的诊断和治疗方法具有重要意义。方法：儿童哮喘数据集从Gene Expression Omnibus （GEO）数据库中获取。采用Limma包筛选哮喘儿童与健康人之间的差异表达基因（DEGs）。使用GO、KEGG和GSEA分析对deg进行进一步分析。通过PPI分析发现与儿童哮喘相关的枢纽基因。从DrugBank数据库中获取药物靶中心基因。使用Autodock vina来探索靶向药物与枢纽基因的结合能力。结果：共从GSE152004和GSE65204数据集中筛选出80个deg。细胞因子-细胞因子受体相互作用是共享DEGs功能富集分析确定的关键途径。通过构建的PPI网络共鉴定出4个枢纽基因（CCL26、CXCR6、IL18RAP和CCL20），其中CXCR6、IL18RAP和CCL20在儿童哮喘数据集中显著减少。然而，CCL26在儿童哮喘数据集中显著升高。另外，使用额外的数据集GSE19187和GSE240567进行验证。最终，靶向中枢基因的药物（西咪替丁、头孢克洛和异丙酚）具有良好的组合能力。结论：我们已经确定CCL26、CXCR6、IL18RAP和CCL20可能在儿童哮喘的进展中起关键作用，因此有可能成为靶向治疗以增加儿童哮喘的治疗选择。整合、创新和洞察声明：细胞因子-细胞因子受体相互作用是儿童哮喘发生的关键途径。中心基因（CCL26、CXCR6、IL18RAP和CCL20）影响儿童哮喘的发展。以中枢基因为靶点的药物（西咪替丁、头孢克洛和异丙酚）具有良好的组合能力。

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Screening of cytokines-cytokine receptor-associated genes in childhood asthma based on bioinformatics.

Purpose: To develop efficient diagnostic and treatment approaches, gaining an in-depth knowledge of the molecular mechanisms and potential targets causing childhood asthma is of utmost significance.

Methods: Childhood asthma datasets were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between asthmatic child and healthy people were screened by the Limma package. DEGs were subjected to further analyses utilizing GO, KEGG and GSEA analysis. The hub genes associated with childhood asthma were discovered by PPI analysis. The drugs target hub genes were accessed from the DrugBank database. Autodock vina was used to explore the binding ability of targeted drugs to hub genes.

Results: Total 80 DEGs were selected from GSE152004 and GSE65204 datasets. The cytokine-cytokine receptor interaction was the key pathway identified by functional enrichment analysis of shared DEGs. A total of 4 hub genes (CCL26, CXCR6, IL18RAP and CCL20) were identified by the constructed PPI network, among which CXCR6, IL18RAP and CCL20 were significantly decreased in childhood asthma datasets. Whereas, the CCL26 was significantly increased in childhood asthma datasets. Additionally, the extra dataset GSE19187 and GSE240567 were employed for validation. Ultimately, drugs (Cimetidine, Cefaclor and Propofol) that target hub genes have favorable combination ability.

Conclusions: We have determined that CCL26, CXCR6, IL18RAP and CCL20 might have crucial involvement in the advancement of childhood asthma, thus having the potential to be targeted therapeutically in order to enhance treatment choices for childhood asthma. Statement of Integration, Innovation and Insight: The cytokine-cytokine receptor interaction is a key pathway in the occurrence of childhood asthma. The hub genes (CCL26, CXCR6, IL18RAP and CCL20) affect the development of childhood asthma. The drugs (Cimetidine, Cefaclor and Propofol) that target hub genes have favorable combination ability.

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来源期刊

Integrative Biology 生物-细胞生物学

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Integrative Biology publishes original biological research based on innovative experimental and theoretical methodologies that answer biological questions. The journal is multi- and inter-disciplinary, calling upon expertise and technologies from the physical sciences, engineering, computation, imaging, and mathematics to address critical questions in biological systems. Research using experimental or computational quantitative technologies to characterise biological systems at the molecular, cellular, tissue and population levels is welcomed. Of particular interest are submissions contributing to quantitative understanding of how component properties at one level in the dimensional scale (nano to micro) determine system behaviour at a higher level of complexity. Studies of synthetic systems, whether used to elucidate fundamental principles of biological function or as the basis for novel applications are also of interest.