Ulrike K. R. Kammann, Verena Töpker, Jörn Peter Scharsack
{"title":"通过对三种比目鱼的尿液和胆汁进行分析，追踪波罗的海西部倾倒弹药中的爆炸性污染物","authors":"Ulrike K. R. Kammann, Verena Töpker, Jörn Peter Scharsack","doi":"10.1186/s12302-025-01074-0","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Dumped munitions in German coastal waters, particularly the explosive 2,4,6-trinitrotoluene (TNT), may pose significant environmental risks. TNT and its metabolites, such as 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT), contaminate marine organisms, including fish. These TNT metabolites bioaccumulate in fish tissues, serving as markers for environmental monitoring. Traditionally, fish bile has been a primary matrix to investigate TNT exposure; however, the present study is the first to explore the use of fish urine to detect TNT metabolites. Three flatfish species, common dab (<i>Limanda limanda</i>), European plaice (<i>Pleuronectes platessa</i>), and European flounder (<i>Platichthys flesus</i>) from three regions with munition dumping sites in the Western Baltic Sea were investigated.</p><h3>Results</h3><p>Differences in the levels of contamination at the dumping sites are reflected in the concentrations of explosives found in the urine and bile. Fish from the Kolberger Heide dumping site in Kiel Bight exhibited the highest concentrations of explosive contaminants. In individual dab, contamination with 2-ADNT was recorded up to 26.356 ng/ml in bile and 36.120 ng/ml in urine. Concentrations of 4-ADNT ranged up to 95.908 ng/ml in bile and 26.877 ng/ml in urine. The patterns of TNT metabolites in urine and bile varied, and the concentrations of these metabolites in urine and bile did not always correspond in individual fish. However, the different mean contamination levels in the three regions were reflected in both: urine and bile. Contamination levels of explosives in the three regions decreased in the order Kiel > Schlei > Lübeck.</p><h3>Conclusions</h3><p>TNT metabolites were detected in fish urine for the first time. Urine and bile can serve as useful matrices to assess environmental exposure of fish to TNT. Additionally, dab, plaice and flounder can be utilized in studies focusing on the analysis of explosives in bile or urine. The present study supports the development of fish urine usage for reliable and effective monitoring strategies for explosives.</p></div>","PeriodicalId":546,"journal":{"name":"Environmental Sciences Europe","volume":"37 1","pages":""},"PeriodicalIF":6.0000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s12302-025-01074-0.pdf","citationCount":"0","resultStr":"{\"title\":\"Tracking explosive contaminants from dumped munition in the western Baltic Sea via urine and bile analysis of three flatfish species\",\"authors\":\"Ulrike K. R. Kammann, Verena Töpker, Jörn Peter Scharsack\",\"doi\":\"10.1186/s12302-025-01074-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Dumped munitions in German coastal waters, particularly the explosive 2,4,6-trinitrotoluene (TNT), may pose significant environmental risks. TNT and its metabolites, such as 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT), contaminate marine organisms, including fish. These TNT metabolites bioaccumulate in fish tissues, serving as markers for environmental monitoring. Traditionally, fish bile has been a primary matrix to investigate TNT exposure; however, the present study is the first to explore the use of fish urine to detect TNT metabolites. Three flatfish species, common dab (<i>Limanda limanda</i>), European plaice (<i>Pleuronectes platessa</i>), and European flounder (<i>Platichthys flesus</i>) from three regions with munition dumping sites in the Western Baltic Sea were investigated.</p><h3>Results</h3><p>Differences in the levels of contamination at the dumping sites are reflected in the concentrations of explosives found in the urine and bile. Fish from the Kolberger Heide dumping site in Kiel Bight exhibited the highest concentrations of explosive contaminants. In individual dab, contamination with 2-ADNT was recorded up to 26.356 ng/ml in bile and 36.120 ng/ml in urine. Concentrations of 4-ADNT ranged up to 95.908 ng/ml in bile and 26.877 ng/ml in urine. The patterns of TNT metabolites in urine and bile varied, and the concentrations of these metabolites in urine and bile did not always correspond in individual fish. However, the different mean contamination levels in the three regions were reflected in both: urine and bile. Contamination levels of explosives in the three regions decreased in the order Kiel > Schlei > Lübeck.</p><h3>Conclusions</h3><p>TNT metabolites were detected in fish urine for the first time. Urine and bile can serve as useful matrices to assess environmental exposure of fish to TNT. Additionally, dab, plaice and flounder can be utilized in studies focusing on the analysis of explosives in bile or urine. 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Tracking explosive contaminants from dumped munition in the western Baltic Sea via urine and bile analysis of three flatfish species
Background
Dumped munitions in German coastal waters, particularly the explosive 2,4,6-trinitrotoluene (TNT), may pose significant environmental risks. TNT and its metabolites, such as 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT), contaminate marine organisms, including fish. These TNT metabolites bioaccumulate in fish tissues, serving as markers for environmental monitoring. Traditionally, fish bile has been a primary matrix to investigate TNT exposure; however, the present study is the first to explore the use of fish urine to detect TNT metabolites. Three flatfish species, common dab (Limanda limanda), European plaice (Pleuronectes platessa), and European flounder (Platichthys flesus) from three regions with munition dumping sites in the Western Baltic Sea were investigated.
Results
Differences in the levels of contamination at the dumping sites are reflected in the concentrations of explosives found in the urine and bile. Fish from the Kolberger Heide dumping site in Kiel Bight exhibited the highest concentrations of explosive contaminants. In individual dab, contamination with 2-ADNT was recorded up to 26.356 ng/ml in bile and 36.120 ng/ml in urine. Concentrations of 4-ADNT ranged up to 95.908 ng/ml in bile and 26.877 ng/ml in urine. The patterns of TNT metabolites in urine and bile varied, and the concentrations of these metabolites in urine and bile did not always correspond in individual fish. However, the different mean contamination levels in the three regions were reflected in both: urine and bile. Contamination levels of explosives in the three regions decreased in the order Kiel > Schlei > Lübeck.
Conclusions
TNT metabolites were detected in fish urine for the first time. Urine and bile can serve as useful matrices to assess environmental exposure of fish to TNT. Additionally, dab, plaice and flounder can be utilized in studies focusing on the analysis of explosives in bile or urine. The present study supports the development of fish urine usage for reliable and effective monitoring strategies for explosives.
期刊介绍：
ESEU is an international journal, focusing primarily on Europe, with a broad scope covering all aspects of environmental sciences, including the main topic regulation.
ESEU will discuss the entanglement between environmental sciences and regulation because, in recent years, there have been misunderstandings and even disagreement between stakeholders in these two areas. ESEU will help to improve the comprehension of issues between environmental sciences and regulation.
ESEU will be an outlet from the German-speaking (DACH) countries to Europe and an inlet from Europe to the DACH countries regarding environmental sciences and regulation.
Moreover, ESEU will facilitate the exchange of ideas and interaction between Europe and the DACH countries regarding environmental regulatory issues.
Although Europe is at the center of ESEU, the journal will not exclude the rest of the world, because regulatory issues pertaining to environmental sciences can be fully seen only from a global perspective.
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