J R Dave, S G Culp, L Liu, B Tabakoff, P L Hoffman
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引用次数: 0
摘要
最近的研究表明,免疫反应性催产素(OT)和抗利尿激素(VP)、OT和VP受体以及这两种激素的生理功能存在于包括垂体前叶在内的多种外周组织中。本研究的目的是确定(i) OT和VP基因是否在大鼠睾丸和垂体前叶中表达,以及(ii)已知可以改变下丘脑中OT和VP基因调节的渗透刺激是否会改变这些基因在大鼠睾丸和垂体前叶中的表达。使用寡核苷酸探针(由dr。M. Brownstein和W. Scott Young (NIMH))对应的VP基因或OT基因和人类OT和VP基因的特定部分(由J. Battey博士提供,NCI)在pGEM-3核探针系统中亚克隆,以及Northern blot和slot blot技术,在大鼠睾丸和垂体前叶中发现了OT和VP mrna。成年雄性大鼠(SD)在不饮水或以2%盐溶液作为唯一饮水来源72小时后,下丘脑、垂体前腺和睾丸中OT和VP mRNA水平均升高。我们的数据表明,睾丸和垂体前叶也可能是OT和VP的合成位点,同样的刺激可能在不同的组织中调节这些基因。
Regulation of vasopressin and oxytocin synthesis in anterior pituitary and peripheral tissues.
Recent studies have demonstrated the presence of immunoreactive oxytocin (OT) and vasopressin (VP), OT and VP receptors and physiological functions for these two hormones in a variety of peripheral tissues, including anterior pituitary gland. The objectives of this study were to determine if (i) OT and VP genes are expressed in rat testis and anterior pituitary gland and (ii) if osmotic stimulation known to modify the regulation of OT and VP genes in hypothalamus, would modify the expression of these genes in rat testis and anterior pituitary gland. Using oligonucleotide probes (courtesy of Drs. M. Brownstein and W. Scott Young, NIMH) corresponding to the VP gene or OT gene and specific fractions of human OT and VP genes (courtesy of Dr. J. Battey, NCI) subcloned in the pGEM-3 riboprobe system, and Northern blot and slot blot techniques, OT and VP mRNAs were found in rat testis and anterior pituitary gland. When adult male rats (SD) were either deprived of drinking water or offered 2% salt solution as a sole source of drinking fluid for 72 hrs, both OT and VP mRNA levels were increased in hypothalamus, anterior pituitary gland and testis. Our data suggest that testis and anterior pituitary gland could also be sites of synthesis of OT and VP and that the same stimulus may regulate these genes in various tissues.