基于全基因组重测序的带梗狐尾草性别连锁标记的开发与应用。

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Genes & genetic systems Pub Date : 2025-05-01 Epub Date: 2025-02-27 DOI:10.1266/ggs.24-00182

An-Ning Li, Zhi-Li Zhou, Xi-Long Wang, Xue-Mei Wen, Yan-Li Tu, Li-Hua Meng

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引用次数: 0

摘要

性别特异性DNA标记是雌雄异株生物性别鉴定和性别控制育种的有效工具。雌雄异株带梗草（Herpetospermum peduncullosum）的种子具有药用价值，而种子性别连锁标记的开发是提高雌性植株数量的关键。本研究基于20个雄性和24个雌性的全基因组重测序，筛选了性别特异性标记，并利用简单PCR方法在4个种群的80个个体中验证了505 bp的雄性特异性DNA片段。该结果为长柄花幼苗的性别鉴定提供了可靠的雄性特异性标记。

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Development and application of a sex-linked marker for Herpetospermum pedunculosum based on whole-genome resequencing.

Sex-specific DNA markers are effective tools for sex identification and sex-controlled breeding of dioecious organisms. The seeds of the dioecious Herpetospermum pedunculosum are utilized in traditional Chinese medicine, and the development of sex-linked markers for seedlings is crucial for enhancing the number of female plants. In this study, we screened sex-specific markers based on whole-genome resequencing of 20 male and 24 female H. pedunculosum individuals, and validated a male-specific DNA fragment of 505 bp among 80 individuals from four populations using simple PCR. The findings provide a reliable male-specific marker for the sex identification of H. pedunculosum seedlings.

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