从离子色谱法收集生物活性化合物的部分：不再是不可能的任务

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2025-02-21 DOI:10.1016/j.mex.2025.103243

Hans S.A. Yates , James F. Carter , Mary T. Fletcher , Viviene S. Santiago , Ondrea Thompson , Natasha L. Hungerford

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引用次数: 0

摘要

本文演示了利用改进的离子色谱仪对新型糖海藻糖的组分收集。先前发表的用于分析海藻糖的离子色谱（IC）方法增加了一个抑制因子和专用的开关阀单元。然后运行无刺蜂蜂蜜样品，遵循三个主要步骤：•海藻糖部分的分离•冻干•海藻糖的确认该方法不仅适用于糖分析，还适用于任何可通过IC分离的生物活性化合物。作者无法在当前发表的文献中找到类似的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

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Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:

•
Separation of trehalulose fraction
•
Lyophilization
•
Confirmation of trehalulose

The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.

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