circRNA 和 RBP 的全转录组分析及其与 GBM 的分子相关性。

IF 4.5 2区 医学 Q1 Biochemistry, Genetics and Molecular Biology
Molecular Oncology Pub Date : 2025-08-01 Epub Date: 2025-02-26 DOI:10.1002/1878-0261.70005
Julia Latowska-Łysiak, Żaneta Zarębska, Marcin P Sajek, Adriana Grabowska, Alessia Buratin, Paweł Głodowicz, Julia O Misiorek, Konrad Kuczyński, Stefania Bortoluzzi, Marek Żywicki, Jan G Kosiński, Agnieszka Rybak-Wolf, Rafał Piestrzeniewicz, Anna M Barciszewska, Katarzyna Rolle
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引用次数: 0

摘要

胶质母细胞瘤(GBM)是最具侵袭性和致死性的胶质瘤类型,其特征是包括环状rna (circRNAs)在内的非编码rna的异常表达。CircRNAs可能通过与其他分子如rna结合蛋白(rbp)相互作用来影响细胞过程。circRNA和circRNA/RBP复合物的诊断价值在很大程度上仍然未知。为了探索circRNA和RBP转录物在GBM中的表达,我们执行并进一步分析了GBM患者原发和复发肿瘤样本的RNA-seq数据。我们鉴定了原发性肿瘤中circRNA的差异表达、复发性GBM样本中的circRNA进展标记以及RBP基因的表达谱。此外,我们证明了环状rna和rbp在GBM中的临床潜力,并建议它们作为新生组装肿瘤亚型的分层标记物。此外,我们通过实验验证了选定环状rna的亚细胞定位及其与FUS的相互作用。随后,我们发现circARID1A可能在促进GBM细胞增殖中发挥作用。总的来说,我们描述了circRNA-RBP相互作用可能在胶质瘤形成和GBM进展中发挥调节作用,并提供了GBM中的分子参与者列表,以供进一步广泛研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome-wide analysis of circRNA and RBP profiles and their molecular relevance for GBM.

Glioblastoma (GBM) is the most aggressive and lethal type of glioma, characterized by aberrant expression of noncoding RNAs including circular RNAs (circRNAs). CircRNAs may impact cellular processes by interacting with other molecules-like RNA-binding proteins (RBPs). The diagnostic value of circRNA and circRNA/RBP complexes is still largely unknown. To explore circRNA and RBP transcript expression in GBM, we performed and further analyzed RNA-seq data from GBM patients' primary and recurrent tumor samples. We identified circRNAs differentially expressed in primary tumors, the circRNA progression markers in recurrent GBM samples, and the expression profile of RBP genes. Furthermore, we demonstrated the clinical potential of circRNAs and RBPs in GBM and proposed them as stratification markers in de novo assembled tumor subtypes. Additionally, we experimentally validated the subcellular localization of select circRNAs and their interactions with FUS. Subsequently, we showed that circARID1A may play a role in promoting GBM cell proliferation. Overall, we described circRNA-RBP interactions that could play a regulatory role in gliomagenesis and GBM progression and provided a list of molecular players in GBM for further extensive studies.

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来源期刊
Molecular Oncology
Molecular Oncology Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
11.80
自引率
1.50%
发文量
203
审稿时长
10 weeks
期刊介绍: Molecular Oncology highlights new discoveries, approaches, and technical developments, in basic, clinical and discovery-driven translational cancer research. It publishes research articles, reviews (by invitation only), and timely science policy articles. The journal is now fully Open Access with all articles published over the past 10 years freely available.
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