培养基类型和血清添加量对体外培养牛囊胚发育和基因表达的影响。

IF 2.5 4区 生物学 Q3 CELL BIOLOGY
Physiological genomics Pub Date : 2025-04-01 Epub Date: 2025-02-25 DOI:10.1152/physiolgenomics.00142.2024
Camila J Cuellar, Esraa A Ismail, McKenzie L J Haimon, Quinn A Hoorn, Fahong Yu, Maria Belen Rabaglino, Peter James Hansen
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引用次数: 0

摘要

血清通常被添加到培养基中以提高体外牛胚胎的产量。主要目的是验证血清增加囊胚产量的作用,并验证胎牛血清改变囊胚基因表达的假设,这种方式可能影响建立妊娠的能力和胎儿发育失调。使用的培养基是合成输卵管液培养基牛胚胎2 (sofbe2)和商业培养基称为IVFB。在发育第1天或第5天分别添加10%的成牛血清和胎牛血清进行了3个试验。总的来说,血清增加了囊胚的产生。在第5天添加胎牛血清的实验中,测定了囊胚中的基因表达。在SOF-BE2培养基中培养的胚胎血清中有215个差异表达基因,在IVFB培养基中培养的胚胎血清中有194个差异表达基因(调整p值< 0.05,|log2|倍变化> 1)。在两种培养基中,血清中只有24个基因表达相似,包括几种转录因子、印迹基因、小鼠胎儿生长相关的PSAT1和克隆胚胎中失调的基因。血清在很大程度上消除了培养基间基因表达的差异。8个生物标记基因的表达数据也被用来计算先前与胚胎存活相关的胚胎能力指数。血清降低了两种培养基的胚胎能力指数。综上所述,胎牛血清对着床前胚胎的作用包括基因表达的改变,这表明胚胎能力降低,并可能改变胎儿的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interactions between type of culture medium and addition of serum on development and gene expression of bovine blastocysts produced in vitro.

Serum is commonly added to culture medium to improve the production of bovine embryos in vitro. The main goals were to verify the actions of serum to increase blastocyst yield and test the hypothesis that fetal bovine serum alters blastocyst gene expression in a manner that could affect competence to establish pregnancy and dysregulate fetal development. Media used were synthetic oviduct fluid medium bovine embryo 2 (SOF-BE2) and a commercial medium from IVF Biosciences termed here as IVFB. Three experiments were conducted in which either adult or fetal bovine serum (10%, vol/vol) was added at day 1 or 5 of development. Overall, serum increased blastocyst production. Gene expression in blastocysts was measured in the experiment in which fetal bovine serum was added at day 5. Serum resulted in 215 differentially expressed genes for embryos cultured in SOF-BE2 and 194 genes for embryos cultured in IVFB (adjusted P value of <0.05 and a |log2| fold change >1). Only 24 genes were regulated by serum similarly for both media, including several transcription factors, imprinted genes, PSAT1 implicated in fetal growth in mice, and genes dysregulated in cloned embryos. Serum largely eliminated differences in gene expression between media. Expression data on eight biomarker genes were also used to calculate an embryo competence index previously related to embryo survival. Serum lowered the embryo competence index for both media. In conclusion, actions of fetal bovine serum on the preimplantation embryo include changes in gene expression indicative of reduced embryo competence and possible alterations in fetal development.NEW & NOTEWORTHY Serum is commonly added to the culture medium to improve the production of bovine embryos in vitro, but its molecular consequences for the resultant embryo are unclear. Here, we showed that blastocysts produced in serum experienced changes in gene expression, including transcription factors and imprinted genes. An embryo competence index that predicts embryo's ability to establish pregnancy based on gene expression was reduced by serum, suggesting serum can reduce embryo survivability.

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来源期刊
Physiological genomics
Physiological genomics 生物-生理学
CiteScore
6.10
自引率
0.00%
发文量
46
审稿时长
4-8 weeks
期刊介绍: The Physiological Genomics publishes original papers, reviews and rapid reports in a wide area of research focused on uncovering the links between genes and physiology at all levels of biological organization. Articles on topics ranging from single genes to the whole genome and their links to the physiology of humans, any model organism, organ, tissue or cell are welcome. Areas of interest include complex polygenic traits preferably of importance to human health and gene-function relationships of disease processes. Specifically, the Journal has dedicated Sections focused on genome-wide association studies (GWAS) to function, cardiovascular, renal, metabolic and neurological systems, exercise physiology, pharmacogenomics, clinical, translational and genomics for precision medicine, comparative and statistical genomics and databases. For further details on research themes covered within these Sections, please refer to the descriptions given under each Section.
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