耳念珠菌甘露糖基转移酶MTN5在抗真菌治疗和宿主生物膜群落保护中的双重作用。

IF 5.1 1区生物学 Q1 MICROBIOLOGY

mBio Pub Date : 2025-04-09 Epub Date: 2025-02-25 DOI:10.1128/mbio.00346-25

Robert Zarnowski, Mark V Horton, Chad J Johnson, Por Choua Vang, Jeremy Uram, Liyanage Devthilini Pasasum Fernando, Jiri Vlach, Christian Heiss, Parastoo Azadi, Jeniel E Nett, David R Andes

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引用次数: 0

摘要

筛选甘露糖基转移酶家族突变体，发现MNT5在金黄色葡萄球菌生物膜耐药和中性粒细胞逃逸中的重要性。对mnt5突变体基质和细胞壁的生化分析发现甘露聚糖结构发生了变化。通过细胞外囊泡传递野生型基质，恢复mnt5∆的抗性和基质。对mnt5∆细胞壁的分析显示，甘露聚糖减少，细胞表面葡聚糖和几丁质代偿性增加，表明mnt5在甘露聚糖掩盖病原体相关分子模式中的作用。重要性：耳念珠菌的耐药性与生物膜耐药和免疫逃避有关。MNT5编码的甘露糖基转移酶对于两种表型都是必需的，并且可能作为有用的治疗靶点。

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Dual function of Candida auris mannosyltransferase, MNT5, in biofilm community protection from antifungal therapy and the host.

Screen of mutants from a mannosyltransferase family identified the importance of MNT5 for C. auris biofilm drug resistance and neutrophil evasion. Biochemical analysis of the mnt5∆ mutant matrix and cell wall identified alterations in the mannan structures. Resistance and matrix for mnt5∆ were restored with delivery of wild-type matrix via extracellular vesicles. Analysis of the mnt5∆ cell wall revealed a reduction in mannan and compensatory increase in cell surface glucan and chitin, suggesting a role for MNT5 in mannan masking of pathogen-associated molecular patterns.

Importance: C. auris recalcitrance is linked to biofilm drug resistance and immune evasion. The mannosyltransferase encoded by MNT5 is necessary for both phenotypes and may serve as a useful therapeutic target.

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来源期刊

mBio MICROBIOLOGY-

CiteScore

10.50

自引率

3.10%

发文量

762

审稿时长

1 months

期刊介绍： mBio® is ASM''s first broad-scope, online-only, open access journal. mBio offers streamlined review and publication of the best research in microbiology and allied fields.