海洋浮游植物对体外微生物组的组装施加了强大的选择压力,但漂移是主要的过程。

IF 5.1 Q1 ECOLOGY
ISME communications Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI:10.1093/ismeco/ycaf001
Sergio E Morales, Sven P Tobias-Hünefeldt, Evelyn Armstrong, William S Pearman, Kirill Bogdanov
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引用次数: 0

摘要

众所周知,浮游植物是调节海洋群落组装过程的生态系统工程师,但它们控制微生物组的普遍性和程度尚不清楚。我们使用体外培养和16S核糖体RNA基因扩增子测序来测试南太平洋和南太平洋优势浮游植物对浮游植物繁殖响应的组装过程和群落演替的影响。在多样性降低的培养物中生长的浮游植物或在外源添加微生物组的情况下生长的浮游植物多样性降低,表明环境过滤。在所有培养条件下,群落特征都是不同的,进一步证实了浮游植物对特定微生物组的强烈选择。对每种培养条件下的核心生物、丰度生物和稀有生物的分析表明,在外源添加浮游植物的条件下,核心生物富集的反应是保守的。通过浮游植物生长阶段的进展,首先选择稀有和丰富的生物,在指数阶段增加对核心成员的选择,在死亡阶段放松选择,如在仅微生物组对照的孵育过程中所见。令人惊讶的是,选择过程量化表明,在所有条件和生长阶段,漂移是主导过程,其余的是同质选择和分散限制。总之,利用南大洋衍生的模式生物,我们证实了浮游植物在群落聚集中的作用,但也证明了随机过程仍然主导着群落选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Marine phytoplankton impose strong selective pressures on in vitro microbiome assembly, but drift is the dominant process.

Phytoplankton are known ecosystem engineers that modulate ocean community assembly processes, but the universality and extent of their microbiome control remains unclear. We used in vitro incubations and 16S ribosomal RNA gene amplicon sequencing to test the influence of Southern and South Pacific oceans dominant phytoplankton on assembly processes and community successions in response to phytoplankton blooms. Phytoplankton grown with reduced-diversity cultures or supplemented with exogenously added microbiomes showed reduced diversity, suggesting environmental filtering. Community profiles were distinct under all culture conditions, further confirming strong selection for specific microbiomes based on phytoplankton. Analysis of core, abundant, and rare organisms in each culture condition showed a conserved response in which core organisms were enriched under conditions of exogenously added phytoplankton. Progression through phytoplankton growth phases selected first for rare and abundant organisms, with increased selection for core members during the exponential phase and relaxing of selection during the death phase, as seen throughout incubations for microbiome-only controls. Surprisingly, selection process quantification identified drift as the dominant process across all conditions and growth phases, with homogenous selection and dispersal limitation accounting for the remainder. Altogether, using Southern Ocean-derived model organisms we confirmed the role phytoplankton play in community assembly but also demonstrated that stochastic processes still predominately drive community selection.

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